Isolation and primary identification of a new nitrogng - fixation arthrobacter strain 一種有固氮能力的節(jié)桿菌菌株的分離和初步鑒定
Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169 將hyucdna片段連接到真核表達(dá)載體ppic3 . 5k上,經(jīng)bgl酶切線性化,通過peg法轉(zhuǎn)化導(dǎo)入畢赤酵母gs115感受態(tài)細(xì)胞,利用g418抗性篩選得到12個插入多拷貝目的基因的轉(zhuǎn)化子。
Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids 節(jié)桿菌( arthrobacter ) bt801是由軍事醫(yī)學(xué)科學(xué)院生物工程研究所保存的l -乙內(nèi)酰脲酶產(chǎn)酶菌株, puc18 - 169是由本室構(gòu)建的含有節(jié)桿菌bt801的l -乙內(nèi)酰脲酶完整操縱子序列的亞克隆質(zhì)粒。
The l - n - carbamoylase of arthrobacter bt801 , coded by the hyuc gene , is the rate - limiting and the only stereoselective enzyme . hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and , at the same time , activity expression was obtained in e . coli and pichia pastor is gs115 respectively 節(jié)桿菌bt801的乙內(nèi)酰脲水解酶催化乙內(nèi)酰脲類化合物水解開環(huán), n -氨甲酰基氨基酸水解酶是該菌乙內(nèi)酰脲酶系中惟一具有立體選擇性的酶,也是整個反應(yīng)體系的限速酶。
百科解釋
Arthrobacter (from the Greek, "jointed small stick”) is a genus of bacteria that is commonly found in soil. All species in this genus are Gram-positive obligate aerobes that are rods during exponential growth and cocci in their stationary phase.