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antigen-binding in Chinese

Pronunciation:
How to pronounce "antigen-binding""antigen-binding" in a sentence

Translationmobile phoneMobile

  • 抗原-結合的

Examples

  • Cloning and expression of fragments of fibronectin to screen hepatitis b virus surface antigen - binding domains
    纖連蛋白的分段表達及與乙肝表面抗原相互作用研究
  • After dissolving inclusion bodies , renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ) , the fusion protein trx - scfv of electrophoretic purity was obtained , they still possessed antigen - binding activity
    經變性復性,用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體,在一升的發(fā)酵液中得到78mg的重組蛋白。
  • Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
    El舊a拐成惴現2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ,均高出附性對照( 0
  • Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
    X陽性克到駛知烘扳原kbbjg )濁對陽性克隆進行限制隴臥賜析( uwi和hedlll )鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率,從中j ) ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用于進一涉研究。
  • ( 4 ) in artificial pepsin digestive system with ph2 . 0 , due to the acid denaturation , the pigg was hydrolyzed wholly , in the system with ph3 . 0 and 4 . 0 , although not all entirely pigg could be detected , but the pigg antibody activities against e . coli still existed without changing , this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process
    ( 4 )在ph2 . 0的人工胃液消化體系中,由于酸變性pigg全部水解;在ph3 . 0和ph4 . 0的人工胃液消化體系中,盡管檢測不到所有的pigg ,但pigg仍可與e . coli特異性結合且凝集價未發(fā)生變化,表明在消化過程中具有抗原結合活性的f ( ab ) _ 2片段仍可完整地保留下來。
  • Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養(yǎng)的mg _ 7雜交瘤細胞中提取并分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區(qū)基因,并通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態(tài)tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估m(xù)g _ 7重組噬菌體抗體庫的容量和重組率。
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