One positive clone has been gained from the library screened by m4g3 mcab 3 .文庫擴增后滴度達到1
Mcab monoclonal antibody 單克隆抗體
The average csfv titer of samples is 1 : 32 by indirect elisa , but 1 : 4 dilution is used in practice 通過方陣試驗找出了mcab和血清多抗( pcab )的最佳工作稀釋度。
Sds - page and western - blot assay show that the expressed product was 29kda and could be recognized by anti - 6his mcab Page和westernblot鑒定，獲得原核表達的mbl蛋白。
The ig class is iggi . mcab of at - iii provides very efficient tools for the detection and purification of at - iii 獲得三株分泌抗人抗凝血酶的雜交瘤細胞株d5 、 e2 、 f6 ，免疫球蛋白的類型為igg _ 1型。
After detection , 6 igm type and 4 igg1 type mcab were identified , and the all igg type mcab can capture xhfv antigen with antigen capture elisa but only 2 of the 6 igm mcab did 經檢測， 10株上述單抗中， 4株屬于igg1型， 6株屬于igm型單抗； igg型單抗可全部用于捕捉xhf病毒抗原， igm型單抗不能全部用于捕捉xhf病毒抗原。
A synthesised peptide which contain a partial sequence amino acid residues was used in i - elisa , the result is also positive , it means that the amino acid residues 28 - 35 of e2 protein is likely a linear epitope recognized by mcab all 從而證明核心序列能模擬a11所針對的抗原表位。研究結果提示單抗a11所針對的抗原表位位于e2蛋白的28 ? 35位氨基酸。該區(qū)域可能是csfve2一個新的抗原表位。
Methods : sandwich elisa assay was used , w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody . the second antibody - hrp conjugate was added for coloration . standard curve was obtained by shla - i standard reagent in serial dilution . the amount of shla - i in the samples was determined : 1 方法：以w6 32包被酶標板，捕捉樣品中可溶性hla ，加入一抗2m抗體，再加酶標二抗及底物顯色。根據(jù)可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線： 1
Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c 在對噬菌體環(huán)七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優(yōu)勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，并且能夠阻斷tnf與受體的結合，提示篩選得到的環(huán)七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫(yī)大學顧士學位論文合的特性，為tnfa表位模擬肽。
In this experiment , to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all , which had been prepared in previous experiment , the mcab all was raised in mice and followed by purification , the concentration of protein was assayed by using the bca protein assay kit 本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據(jù)。
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