recombinant bacteria in Chinese
Translation
Mobile
- 重組細(xì)菌
- "recombinant" in Chinese : n. 【遺】重組器官,復(fù)合器官。
- "bacteria" in Chinese : n. pl. (sing. Bacterium ) 1. ...
- "recombinant deficient mutant of bacteria" in Chinese : 細(xì)菌重組缺陷突變型
- "recombinant" in Chinese : n. 【遺】重組器官,復(fù)合器官。
- "bacteria" in Chinese : n. pl. (sing. Bacterium ) 1.細(xì)菌。 2.〔美俚〕 ...
- "alteplase recombinant" in Chinese : 重組阿替普酶
- "bacterial recombinant" in Chinese : 細(xì)菌重組
- "biparental recombinant" in Chinese : 二親重組體
- "dna recombinant" in Chinese : dna重組
- "library of recombinant" in Chinese : 重組基因文庫
- "patch recombinant" in Chinese : 片段重組體
- "plasmid recombinant" in Chinese : 重組體質(zhì)粒
- "recombinant chromosome" in Chinese : 重組染色體
- "recombinant clones" in Chinese : 重組體克隆
- "recombinant dna" in Chinese : 【生物化學(xué)】重組脫氧核糖核酸。
Examples
- These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria . the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit , and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128 , 000
從制備性聚丙烯酰胺凝膠中回收融合蛋白,用mtt法測定重組蛋白刺激小鼠脾淋巴母細(xì)胞增殖反應(yīng)活性,并以重組蛋白免疫家兔制備抗豬il - 6滴度為128 , 000的高免兔血清。 - The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23 . 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析確證重組質(zhì)粒在大腸桿菌中能正確轉(zhuǎn)錄; sds - page分析證明誘導(dǎo)重組質(zhì)粒表達(dá)了融合蛋白,分子量約為50kd ,與預(yù)期的26kd的gst帶和23 . 6kd的草魚生長激素基因編碼蛋白質(zhì)構(gòu)成的融合蛋白大小一致。 - The mrna and protein expression were assayed by rt - pcr and sds - page . the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )
以iptg誘導(dǎo)融合蛋白表達(dá),經(jīng)rt - pcr檢測發(fā)現(xiàn)740bp的特異性il - 6條帶;通過sds - page分析,在49kd處出現(xiàn)gst ? pil6融合蛋白條帶,其表達(dá)量占總細(xì)菌蛋白量的30 ,證明豬il - 6基因得到了正確轉(zhuǎn)錄和表達(dá)。 - The expression conditions of e2 gene in p . pastoris were optimized , the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7 . 5 and 8 . 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e . coli the expression vector pproex - htb respectively , the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e . coli competent bacteria respectively , the recombinant bacteria could express the major antigen region of e2 gene , the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達(dá): pcr擴(kuò)增出當(dāng)前豬瘟流行野毒株,中國豬瘟兔化弱毒( c株)兔脾組織毒ez基因的主要抗原區(qū),將其克隆到原核大腸桿菌表達(dá)載體pproex htb中誘導(dǎo)表達(dá),經(jīng)sds page檢測表明,重組質(zhì)粒能表達(dá)ez基因主要區(qū)蛋白, westernblot檢測表明,誘導(dǎo)表達(dá)蛋白與豬瘟陽性血清發(fā)生特異性反應(yīng),表達(dá)量為35和38 ,可用于基因工程診斷抗原。
Other Languages
Related
"recombinant" in Chinese, "recombinant rna" in Chinese, "recombinant somatropin" in Chinese, "recombinant vaccine" in Chinese, "recombinant dna" in Chinese, "biparental recombinant" in Chinese, "recombinant soluble" in Chinese, "alteplase recombinant" in Chinese, "triparental recombinant" in Chinese, "recom redstone computer" in Chinese, "recombed top" in Chinese, "recombinagen" in Chinese, "recombinagenic" in Chinese, "recombinant chromosome" in Chinese, "recombinant clones" in Chinese, "recombinant deficient mutant" in Chinese, "recombinant deficient mutant of bacteria" in Chinese, "recombinant deficient mutant of phage" in Chinese,
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