√在线天堂中文最新版网,97se亚洲综合色区,国产成人av免费网址,国产成人av在线影院无毒,成人做爰100部片

簡(jiǎn)體版 繁體版
Login Sign Up

recombinant phage in Chinese

Pronunciation:
How to pronounce "recombinant phage""recombinant phage" in a sentence

Translationmobile phoneMobile

  • 重組噬菌體

Examples

  • Tg1 and 15 separate colonies were assayed by elisa . the recombinant phages which were rescued from colony 12 showed the highest affinity to immobilized hcg . the scfv gene was inserted into the reconstructed pet - 21a and expressed in
    用elisa方法進(jìn)行檢測(cè)，從結(jié)合力最強(qiáng)的克隆中分離單鏈抗體基因，插入經(jīng)改造的高效表達(dá)載體pet - 21a中，轉(zhuǎn)化大腸桿菌，誘導(dǎo)表達(dá)。
  • The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
    首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應(yīng)用重組噬菌體抗體庫(kù)技術(shù)，構(gòu)建了一個(gè)針對(duì)牛精子的噬菌體抗體文庫(kù)。
  • The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
    取陽(yáng)性重組噬菌體抗體克隆株pcsa1 ， pcr擴(kuò)增其scfv基因，篩選重組子進(jìn)行序列測(cè)定，發(fā)現(xiàn)其序列符合小鼠抗體基因的一般特征，并且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區(qū)序列的同源性達(dá)80以上；推測(cè)pcsa1scfv針對(duì)的抗原是磷酸膽堿類(lèi)物質(zhì)。
  • Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
    X陽(yáng)性克到駛知烘扳原kbbjg ）濁對(duì)陽(yáng)性克隆進(jìn)行限制隴臥賜析（ uwi和hedlll ）鑒定三用競(jìng)爭(zhēng)elisatoljmg7重組噬菌體抗體性克隆對(duì)mg7單扶與其相應(yīng)抗原結(jié)合忙的喇率，從中j ） ed出對(duì)mg7單抗與期?？乖Y(jié)合有抑余j效應(yīng)的克隆用于進(jìn)一涉研究。
  • Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii , the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
    3 mg _ 7重組噬菌體抗體庫(kù)的淘篩用m13ko7輔助噬菌體感染轉(zhuǎn)化菌，以挽救出噬菌體形式的抗體庫(kù)；用高表達(dá)mg _ 7抗原的胃癌細(xì)胞系kato對(duì)抗體庫(kù)進(jìn)行三輪淘篩；用elisa篩檢呈現(xiàn)有mg _ 7scfv的噬菌體單克隆。
  • Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information . using pcr , the nucleotide acid fragments encoding these putative epitopes were amplified , then cloned into the expression vector miske . the positive recombinant phage displying the epitopes were found out by using pcr , sequencing and the determination of phage plaque titer
    運(yùn)用goldenkey分子生物學(xué)軟件對(duì)prrsvbj - 4結(jié)構(gòu)蛋白的抗原表位及其二級(jí)結(jié)構(gòu)進(jìn)行了分析和比較，從中篩選13段顯示表位特征的氨基酸殘基序列，用pcr技術(shù)擴(kuò)增相應(yīng)的核苷酸片段，將其插入到噬菌體表達(dá)載體m13ke ，結(jié)果預(yù)測(cè)的13個(gè)表位可在噬菌體表面得以展示。
  • Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
    Mg _ 7重組噬菌體抗體庫(kù)的構(gòu)建及鑒定從培養(yǎng)的mg _ 7雜交瘤細(xì)胞中提取并分離mrna ，反轉(zhuǎn)錄成cdna ；利用pcr分別擴(kuò)增mg _ 7單抗的重鏈及輕鏈可變區(qū)基因，并通過(guò)? dna連接子將二者連接起來(lái)形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產(chǎn)物轉(zhuǎn)化感受態(tài)tg1大腸桿菌，制備細(xì)菌形式的mg _ 7重組噬菌體抗體庫(kù)；通過(guò)菌落計(jì)數(shù)和限制性酶切分析( ecor和hind )評(píng)估m(xù)g _ 7重組噬菌體抗體庫(kù)的容量和重組率。
What is the meaning of recombinant phage in Chinese and how to say recombinant phage in Chinese? recombinant phage Chinese meaning, recombinant phage的中文，recombinant phage的中文，recombinant phage的中文，translation, pronunciation, synonyms and example sentences are provided by ichacha.net.