- 克隆擴(kuò)充
- 克隆擴(kuò)增
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clonal expansion中文什么意思
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- Upon the differentiation induction of 3t3 - l1 preadipocytes by hormone cocktail containing 1 - isobutyl - 3 - methylxanthine , dexamethasone and insulin , the preadipocytes undergo ~ 2 rounds of mitotic clonal expansion , which just precedes the adipogenic gene expression program and has been thought to be an essential early step for differentiation initiation
誘導(dǎo)分化的早期細(xì)胞會(huì)進(jìn)行約兩輪的細(xì)胞分裂，被稱為克隆擴(kuò)增?？寺U(kuò)增以前一直被認(rèn)為可能有利于染色體的重構(gòu)以易化分化所需要的基因表達(dá)，因此克隆擴(kuò)增可能是3t3 l1前脂肪細(xì)胞分化所必需的過程。 - Disruption of lipid rafts and caveolae by depleting cellular cholesterol with cholesterol - binding reagent , - methylcyclodextrin or filipin , blocks the igf - 1 receptor signaling in 3t3 - l1 preadipocyte . both hormone induced adipocyte differentiation and mitotic clonal expansion are inhibited by lipid rafts and caveolae disruption . however , a nonspecific lipid binding reagent , xylazine , does not affect adipocyte differentiation or mitotic expansing
利用膽固醇結(jié)合試劑，如甲基環(huán)糊精和菲律賓菌素，減少細(xì)胞膽固醇的含量，破壞細(xì)胞膜上脂質(zhì)筏/質(zhì)膜微囊的結(jié)構(gòu)，抑制了igf - 1受體信號(hào)誘導(dǎo)的3t3 - l1前脂肪細(xì)胞的分化以及克隆擴(kuò)增。