whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva . it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells . another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b . belcheri tsingtauense s004cdna序列編碼一個156個氨基酸殘基組成的蛋白質(zhì),與人的泛素蛋白ubf80具有較高的氨基酸序列同源性(145/156同源),且該蛋白具有泛素蛋白ubf80所共有的兩個結(jié)構(gòu)域,即由72個氨基酸組成的泛素介導(dǎo)的蛋白降解結(jié)構(gòu)域和由48個氨基酸組成的核糖體蛋白s27a結(jié)構(gòu)域。
ranging from 100 to 200 microns across, the width of several human hairs, these microscopic fossils are surprisingly complex and constitute almost a textbook example of a bilaterian, including the three major tissue layers ( the endoderm, mesoderm and ectoderm familiar from high school biology texts ), the presence of a gut with a mouth and anus, and paired coeloms ( body cavities ) surrounding the gut 這些微化石的大小為100~200微米,相當于幾根頭發(fā)的寬度,?們看起來出奇復(fù)雜,而且簡直就像兩側(cè)對稱動物的教科書般,展示了各種特徵:包括三個主要的組織層(內(nèi)胚層、中胚層及外胚層,都是中學(xué)生物課本中熟悉的名詞),前后連接口部與肛門的腸腔,腸腔外還圍繞著成對的體腔。