In the present study , aflp ( amplified fragment length polymorphism ) markers was used to examine population of abies yuanbaoshanensis in order to understand the level of population genetic variation and genetic structure . the result would help to evaluate its evolutionary potentiality and the degree of being endangered and could provide scientific basis for making right protection strategy . high - quality dna was extracted using ctab method from those tender leaves of forty - three fully - developed trees in population abies yuanbaoshanensis 本研究選用一種高效的檢測遺傳變異的分子標(biāo)記? ? aflp技術(shù)來分析元寶山冷杉種群的遺傳多樣性,旨在了解該種群在分布區(qū)內(nèi)的遺傳變異水平和遺傳結(jié)構(gòu)情況;研究結(jié)果將有助于更清楚地認識這一瀕危類群的生存潛力和瀕危程度,而且可以為制定何種挽救和保護措施提供科學(xué)的依據(jù)。
1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out 具體包括以下幾個部分: 1 、針對雁形目鳥類異常復(fù)雜的分類狀況及分類上存在的爭議,根據(jù)雁形目鳥類的形態(tài)學(xué)、行為學(xué)、骨骼學(xué)、角蛋白、線粒體與核dna酶切片段長度多態(tài)、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究,對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統(tǒng)發(fā)生關(guān)系進行綜述,分析系統(tǒng)學(xué)研究中存在的不足,提出了雁形目鳥類分類中急需解決的問題。
All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc ) 所有的樣本通過聚合酶鏈?zhǔn)椒磻?yīng)?限制性片段長度多態(tài)性方法( pcr - rflp )對ahsg基因的第7個外顯子內(nèi)的sac多態(tài)性位點進行基因分型,該多態(tài)性位點為238號氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變?yōu)榻z氨酸( ser , agc ) 。
The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates 本研究通過對豬繁殖與呼吸綜合征病毒( prrsv )國內(nèi)分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析,系統(tǒng)研究了國內(nèi)分離毒株的抗原特性和遺傳學(xué)差異。
Analyse the research of distributing of microbe community and the tendency of the change , disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism , to research the law of change that the microbe community have in composting process , we can get effective and rapid information to filtrate the microorganism during composting process , then accelerate the development of compost technology 摘要對堆肥微生物種群分布及其動態(tài)變化的研究進行了分析,論述了分子生物技術(shù)中的變性梯度凝膠電泳和末端標(biāo)記限制性片段長度多態(tài)性的原理和特點,以及用于研究堆肥微生物的群落結(jié)構(gòu)演變規(guī)律,為分析和篩選堆肥中的微生物提供更加有效、快速的信息,促進堆肥技術(shù)的發(fā)展。