Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province . 2 . the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough 應(yīng)用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現(xiàn)病毒學(xué)反跳時(shí)的血清進(jìn)行ymdd突變株的檢測,并與測序法和mpcr - rflp法比較它們的敏感性、一致性。
The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products . when there were two dna fragments of 363bp and 305bp were produced from a pcr product , the strains were assumed to have a mutation at ser83 , when three fragments of 363bp , 206bp and 99bp were produced , the strains were assumed to have no mutation at the 83or 116 . the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp 本研究重點(diǎn)分析了16株菌對氟喹諾酮類( fqns )藥物的耐藥性,結(jié)果表明: 16株菌對諾氟沙星、環(huán)丙沙星、沙拉沙星、單諾沙星和氧氟沙星等( nor 、 cip 、 sar 、 dan 、 ofl )的耐藥率在31 . 3 - 56 . 3之間。
The dissertation consists of five chapters : in chapter one , the recent progress in molecular approaches in systematic studies of macroalgae e . g . dna extraction , restriction endonuclease fragment length polymorphisms ( rflps ) , random amplified polymorphic dna ( rapd ) , gene sequencing , intersimple sequence jepeats ( issr ) , amplified fragment length polymorphisms ( aflp ) and single strand . conformation polymorphisms ( sscp ) were reviewed 本論文由五部分組成:在第一部分,綜述了大型海藻dna的提取、限制性片段長度多態(tài)性( rflps ) 、隨機(jī)擴(kuò)增多態(tài)性dna ( rapd ) 、核酸序列分析、擴(kuò)增片段長度多態(tài)( aflp ) 、單鏈構(gòu)象多態(tài)( sscp )等分子手段在大型海藻系統(tǒng)學(xué)研究中應(yīng)用的一些進(jìn)展。
Some characteristics of cold - active protease and chitinase were analyzed then . microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences . the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area , as well as the interaction between microbial community and environment , were analyzed based on these studies 通過構(gòu)建沉積物中微生物16srdna克隆文庫,采用pcr - rflp分析、 dna - dna雜交、 16srdna序列測定以及系統(tǒng)發(fā)育分析的方法,研究了兩太平洋“暖池”區(qū)和東太平洋“結(jié)核”區(qū)兩個(gè)不同海區(qū)深海沉積物中的微生物多樣性和群落結(jié)構(gòu)特征及其與環(huán)境的相互關(guān)系,得到了一些與“暖池”區(qū)環(huán)境特點(diǎn)緊密相關(guān)的新發(fā)現(xiàn),新認(rèn)識。