signal peptide造句
例句與造句
- To guide the cam - binding peptide into apoplast space , the signal peptide from brassica campetris pollen coat protein slr1 ? ps was employed . it has total 26 amino acid , and is a very classic form of signal peptide
為了把cam結(jié)合小肽帶到質(zhì)外體，本實(shí)驗(yàn)選擇了野生蕓苔屬植物( brassicacampetris )花粉胞壁蛋白slr1 - bps的信號(hào)肽。 - The zfp28 cdna sequence is 4104 bp and contains a 2076 bp orf that encodes and 868 amino acids protein with an n - terminal signal peptide , two krab domain , and 14 c - termianal c2h2 zinc finger motifs
Zfp28基因長(zhǎng)4076個(gè)堿基，含8個(gè)外顯子，在基因組dna上長(zhǎng)18kb 。它編碼的蛋白質(zhì)全長(zhǎng)868個(gè)氨基酸，含1個(gè)信號(hào)肽， 2個(gè)krab框和14個(gè)c2h2型的鋅指。 - Based on the 5 " end conservative sequence of grb - ast precursor cdna and the adaptor sequence , two gene specific primers ( gsp ) and an adaptor primer ( ap ) were synthesized respectively for the cloning of signal peptide
首先，根據(jù)已報(bào)道的grb - ast前體cdna靠近5端的保守序列，設(shè)計(jì)合成2條基因特異引物，并根據(jù)接頭序列設(shè)計(jì)合成一條接頭引物，用于信號(hào)肽序列的克隆。 - We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0
我們直接將含有4個(gè)內(nèi)含子和自身信號(hào)肽的2 . 4kb全長(zhǎng)人生長(zhǎng)激素基因直接克隆至真核表達(dá)載體pcdna3 . 0 ，然后利用脂質(zhì)體轉(zhuǎn)染家蠶bmn細(xì)胞，瞬時(shí)表達(dá)hgh 。 - Armigera was analysed and then registered in genbank and accession number was af515667 . the cdna encodes a protein with a plltative 2l amino - acid signal peptide and a 425 amino - acid mature protein with molecular mass of 50 kda
連接產(chǎn)物轉(zhuǎn)化jm ，提取質(zhì)粒進(jìn)行酶切及pcr分析，將此連接產(chǎn)物命名為pchto將pcht轉(zhuǎn)化blzi （ de3 ） ，經(jīng)iptg誘導(dǎo)，提取菌體內(nèi)容物sds page電泳。 - It's difficult to find signal peptide in a sentence. 用signal peptide造句挺難的
- To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
運(yùn)用pcr技術(shù)克隆了馬鈴薯蛋白酶抑制劑基因的信號(hào)肽序列，并將其分別連到crylac 、 gfp基因的5 ’端，構(gòu)建植物轉(zhuǎn)化載體p3301ubisigac和p3301ubisiggfp 。 - The gene contains an open reading frame of 1149 nucleotides ( named ap gene , encoding 383 amino acids ) , which contains a signal peptide consisting of 29 residues and a propeptide of 79 residues
該片段全長(zhǎng)1588bp ，包括一個(gè)1152bp的編碼區(qū)序列，編碼全長(zhǎng)為383個(gè)氨基酸殘基的蛋白質(zhì)。根據(jù)軟件分析與同源性比較推測(cè)，該蛋白質(zhì)含有由29個(gè)氨基酸殘基組成的信號(hào)膚， 79個(gè)氨基酸殘基組成的前膚。 - Among the 70 amino acids of ast n - end signal peptide prediction was carried out by using signal ? v2 . 0 program . the result demonstrated that there was a typical signal peptide with the front 30 amino acids ( maprsacvaallraslltvallqappla ra )
用signalpv2 . 0程序?qū)⒃摶蚓幋aast的n端部分的前70個(gè)氨基酸進(jìn)行信號(hào)肽預(yù)測(cè)，結(jié)果表明n端的前30個(gè)氨基酸( maprsacvaallraslltvallqapplara )為一典型的信號(hào)肽序列。 - The cdna contains 947 nucleotides that codes for a precursor protein of 216 amino acids . sequence analysis shows that the n - terminal 1 - 25 amino acid sequence is a predicted signal peptide and other 26 - 216 amino acid sequence is a mature peptide which has no transmembrane domain
Ecbp21cdna全長(zhǎng)947bp ，編碼216個(gè)氨基酸，序列分析顯示其n端1 - 25氨基酸為信號(hào)肽， 26 - 216氨基酸為成熟蛋白肽段，并且不具有跨膜結(jié)構(gòu)域。 - In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence
在用煙草作為生物反應(yīng)器時(shí)，分別將該單鏈抗體靶向細(xì)胞質(zhì)和內(nèi)質(zhì)網(wǎng)。經(jīng)westernblot分析，靶向細(xì)胞質(zhì)中表達(dá)時(shí)，可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。 - It encodes a protein of 865 amino acids with a signal peptide at the n - terminus , of which a polycystic - kidney - disease ( pkd ) - like domain , a triosephosphate - isomerase ( tim ) catalytic region , a receptor - for - egg jelly ( rej ) - like domain and two tandem chitin - binding - domains ( chbds ) locate from the n - to c - terminus
根據(jù)已知幾丁質(zhì)酶基因的dna保守序列，利用人工合成的pcr引物和cb101總dna ，擴(kuò)增出一個(gè)約2 . 6kb的片段并克隆到pbluescript ks載體上。 - ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻譯后水平通過(guò)pcr擴(kuò)增的方式在t - pa基因5端添加了真核分泌信號(hào)肽序列和植物翻譯起始共有序列aaca ，在3端添加了內(nèi)質(zhì)網(wǎng)定位序列kdel ，構(gòu)建了植物表達(dá)載體pbemt 。 - The coding sequence of the two full - length cdnas were cloned by pcr , and inserted into expression vector pmet a between the downstream of a secreting signal peptide and the upstream of 6x histidine in the same reading frame with the coding sequence . the secreting recombinant expression vectors pmet a b / abp2304 and pmet a a / abp780 were constructed and transformed into pmad16 with lici transformation
利用pcr技術(shù)將上述兩個(gè)全長(zhǎng)cdna的編碼區(qū)克隆到表達(dá)載體pmet上，使之位于因子信號(hào)肽序列的下游， 6個(gè)組氨酸殘基序列的上游，且與之同框，分別構(gòu)建成融合蛋白分泌表達(dá)載體pmet b abp2304和pmet a abp780 。 - The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids , which included a signal peptide of 18 amino acids , a proposed propeptide of 171 amino acids , a partial mature protein of 74 amino acids . the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan , but it only contains partial amino acids of mature protein . the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province
結(jié)論： 600bp產(chǎn)物和已報(bào)道的皖南五步蛇纖溶酶金屬蛋白酶氨基酸序列相比較，有90 . 6 %的同源性； 800bp產(chǎn)物中信號(hào)肽及前肽和已報(bào)道的皖南五步蛇金屬蛋白酶很相似，同源性為97 . 9 % ，但僅含有部分金屬蛋白酶成熟肽的氨基酸序列，推測(cè)此片段為廣< wp = 6 >西五步蛇金屬蛋白酶原基因的一部分。 - To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process , a chimeric secretion form of calmodulin binding peptide , which contains a signal peptide , a calmodulin binding domain and a c - myc epitope was constructed . the chimeric gene was introduced into arabidopsis . it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin , which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant . by observing the potential phenotype change of apoplast calmodulin " antisense " plant , the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是采用生理學(xué)手段和藥理學(xué)方法而得出的體外( invitro )實(shí)驗(yàn)結(jié)果，為了取得質(zhì)外體cam在植物生長(zhǎng)發(fā)育過(guò)程中發(fā)揮重要作用的invivo實(shí)驗(yàn)證據(jù)，根據(jù)動(dòng)物中的一些研究方法，本實(shí)驗(yàn)設(shè)計(jì)并構(gòu)建了帶有信號(hào)肽、 cam結(jié)合肽( can小肽) 、 epitope ( c - myc )融合基因的載體，并將融合基因通過(guò)真空滲入法轉(zhuǎn)入擬南芥，預(yù)期過(guò)表達(dá)的融合蛋白將會(huì)被分泌到細(xì)胞外并與質(zhì)外體cam相結(jié)合，這樣就會(huì)抑制質(zhì)外體cam的功能，從而可以構(gòu)建質(zhì)外體cam的“反義”植株，通過(guò)觀察質(zhì)外體cam “反義植株”的表型改變，就可以推斷質(zhì)外體cam在植物生長(zhǎng)發(fā)育過(guò)程中的功能。