structural genes造句
例句與造句
- blastn analysis showed that each of the 10 sequences had no homology with the structural genes or regulatory genes in the anthocyanin biosynthesis . it was suggested that there were some limitations to isolate the specific ast gene by ddrt-pcr
blastn分析表明，這10個(gè)差異表達(dá)的cdna片段與數(shù)據(jù)庫(kù)中花青苷生物合成途徑中的結(jié)構(gòu)基因和調(diào)節(jié)基因序列沒有同源性，表明用ddrt-pcr的方法克隆特定的ast基因有一定的局限性。 - 2 . cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10.3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr
枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應(yīng)條件的優(yōu)化，分別擴(kuò)增得到了生物素操縱子基因的長(zhǎng)距離pcr產(chǎn)物(10.3kb)和3個(gè)分段pcr產(chǎn)物。 - we constructed 3 kinds of shuttle plasmid encoding hcv structural proteins by means of molecular cloning technique; based on it, 3 kinds of adenovirus vectors of hcv structural proteins were packed, screened and identified . we also selected and synthesized 5 kinds of ctl epitope peptides of hcv structural gene which induced 5 kinds of corresponding hcv specific ctl, which were evaluated by experiments in vivo and in vitro
本研究利用分子克隆技術(shù)，構(gòu)建了三種hcv結(jié)構(gòu)蛋白腺病毒載體穿梭質(zhì)粒，并以此為基礎(chǔ)包裝、篩選和鑒定了三種hcv結(jié)構(gòu)蛋白腺病毒表達(dá)載體，選擇并合成了hcv結(jié)構(gòu)基因區(qū)5條ctl表位多肽，并以此誘導(dǎo)了5種hcv特異性ctl，并對(duì)其進(jìn)行了體內(nèi)外的實(shí)驗(yàn)測(cè)定。 - rapifera, light-independent " akamaru " turnip and light-dependent " tsuda " turnip, were used as test material in this experiment . the expression patterns of pal, chs, chi, as, phya, cop1, cip7 and hy5 were analyzed by northern hybridization . pal, chs, chi and as are the principal structural genes correlated with anthocyanin biosynthesis in anthocyanin biosynthesis pathway; phya, cop1, cip7 and hy5 are the genes correlated with light signal transmission in anthocyanin biosynthesis pathway
本研究是以光敏感型津田蕪菁和光不敏感型赤丸蕪菁為試材，利用northern雜交技術(shù)，對(duì)花青素生物合成途徑中與花青素合成相關(guān)的主要結(jié)構(gòu)基因pal、chs、chi和as及光信號(hào)傳遞相關(guān)的基因phya、cop1、cip7和hy5的表達(dá)進(jìn)行了檢測(cè)。 - but such research on virus is still falling behind relatively . in order to probe the malignancy of virus and its toxicology to cell and tissue, to analyze the function of main structural genes of viral genome in pathogencity and immune, to manufacture drugs, which prevent the virus with bacteria and to effective vaccine, genome must be analyzed
而對(duì)病毒這方面的研究相對(duì)滯后，為了探討病毒對(duì)細(xì)胞、組織的毒性作用及其毒理，分析病毒基因組中主要結(jié)構(gòu)基因在致病和免疫中的功能，研制阻斷病毒與細(xì)胞融合的藥物及其有效的疫苗，為其防制提供全新的思路，就必須以基因組的分析為基礎(chǔ)。 - It's difficult to find structural genes in a sentence. 用structural genes造句挺難的
- by using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr-sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated . 3 . the molecular beacon ( mb ) hybridization technique with visual monitoring was established
建立聚合酶鏈反應(yīng)單鏈構(gòu)象多態(tài)性分析inglestrandconformatlonpolymorphlsmanalysisofpol仰erasechainreactionproduction，pcrsscp）方法，初步調(diào)查漢族人群mbl基因ggc54gac突變情況。 - 5 in vitro specific killing experiments showed that spleen cells induced by pi, p3 and p4, which contained higher proportion of cdg + cells and generated better ctl activity, could be introduced to in vivo experiment against hcv 6 we successfully setup a hcv structural gene in vitro cell-transfection system sp2 / 0-hcv, through which also setup a murine model with subcutaneous transplanting tumor of hcv
將誘導(dǎo)的5種v特異性cl，在體外進(jìn)了丁特異性殺傷實(shí)驗(yàn)，證一實(shí)，出n、p3和p4三條多肽誘導(dǎo)的脾糾泡含較高比例的cd。糾1巾d，叫產(chǎn)上較好的ctl殺傷活性，可以用作t細(xì)胞疫苗進(jìn)fi體內(nèi)抗hcv實(shí)驗(yàn)。 - in this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl-cee . the plasmid pfb 1-cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac-cee
本實(shí)驗(yàn)用pcr擴(kuò)增hcv結(jié)構(gòu)區(qū)基因，克隆到桿狀病毒表達(dá)載體pfastbacl中，構(gòu)建成重組轉(zhuǎn)座載體pfb1-cee，轉(zhuǎn)化dh10bac大腸桿菌感受態(tài)細(xì)胞，篩選陽(yáng)性菌落，抽提大分子質(zhì)粒dna，獲得含hcv結(jié)構(gòu)區(qū)基因的重組桿狀病毒穿梭載體bac-cee，脂質(zhì)體介導(dǎo)轉(zhuǎn)染sf9昆蟲細(xì)胞，出現(xiàn)細(xì)胞病變后，收集含有重組桿狀病毒顆粒的培養(yǎng)上消，重新感染sf9細(xì)胞，收集sf9細(xì)胞，進(jìn)行12.5sds-聚丙烯酰胺凝膠電泳，可見表達(dá)的蛋白條帶。 - in this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl-cee . the plasmid pfb 1-cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac-cee
本實(shí)驗(yàn)用pcr擴(kuò)增hcv結(jié)構(gòu)區(qū)基因，克隆到桿狀病毒表達(dá)載體pfastbacl中，構(gòu)建成重組轉(zhuǎn)座載體pfb1-cee，轉(zhuǎn)化dh10bac大腸桿菌感受態(tài)細(xì)胞，篩選陽(yáng)性菌落，抽提大分子質(zhì)粒dna，獲得含hcv結(jié)構(gòu)區(qū)基因的重組桿狀病毒穿梭載體bac-cee，脂質(zhì)體介導(dǎo)轉(zhuǎn)染sf9昆蟲細(xì)胞，出現(xiàn)細(xì)胞病變后，收集含有重組桿狀病毒顆粒的培養(yǎng)上消，重新感染sf9細(xì)胞，收集sf9細(xì)胞，進(jìn)行12.5sds-聚丙烯酰胺凝膠電泳，可見表達(dá)的蛋白條帶。 - the sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared . by computer software, complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic-variant analyses, and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently
通過計(jì)算機(jī)分析軟件，對(duì)我國(guó)ibv地方流行毒株lx4的主要結(jié)構(gòu)基因全序列、分子特征及遺傳變異進(jìn)行分析比較，并初步分析預(yù)測(cè)傳染性支氣管炎病毒主要結(jié)構(gòu)蛋白上可能存在的t細(xì)胞和b細(xì)胞表位。
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