Column is used for normal phase , weak anion - exchanger and specific separation in reversed phase , especially useful for separations of carbohydrates and sugars Aminopropyl型充填材料對醣類之分析特別有用,亦可應用于弱陰離子交換之充填材料及特殊之逆相分離上。
The crude extraction of dry pea seed , which was obtained through marination , homogenization , filtration , centrifugation and other methods , was precipitated by 50 mmol / l ( final concentration ) mgq2 . the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger . single eluting peak containing ferritin was obtained finally 2 、豌豆種子鐵蛋白的純化豌豆種子經(jīng)浸泡、勻漿、過濾、離心等操作得到豌豆鐵蛋白粗提液,再用終濃度為50mmol / l的mgcl _ 2鹽析等處理后,經(jīng)aca _ ( 22 )凝膠過濾柱層析和deae -纖維素52 ( de52 )陰離子交換柱層析等方法進行純化,得到單一的豌豆鐵蛋白洗脫峰。