例句與用法

1. According to these problems , we adopt to the method of mending material , optimize to fermentation media and partly ferment condition . finally , we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified . with the plasmid pbv220 - ifnr , pbv220 - hgfa , pbv220 - hgfb , pbv220 - hpk5 that expresses serve as the model , adopting the biostat - c15l of b . braun company , utilize the method of mending material to ferment , through optimization fermentation media and optimization partly ferment condition ( ventilate quantity , stir speed , mend material speed ) , eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
   以我室構(gòu)建并穩(wěn)定表達的重組質(zhì)粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規(guī)模生產(chǎn)的菌種bl21 ( de3 ) ，該工程菌株連續(xù)傳代100代表達質(zhì)粒不丟失，表達量穩(wěn)定；采用b . braun公司的biostat - c15l自控發(fā)酵罐，運用分批補料技術(shù)分別進行四種工程菌的高密度發(fā)酵，通過優(yōu)化工程菌發(fā)酵的培養(yǎng)基配方及優(yōu)化部分發(fā)酵條件(通氣量、攪拌速度、補料速度) ，最終建立一種適于目的基因高效表達的高密度發(fā)酵工藝模式。