Alfalfa is the best type of hay for a cavy 苜蓿對天天來說是最好的牧草種類
I propose mary cavy as a candidate for the presidency 我提名瑪麗?戴維斯為總裁候選人。
But uh , rat - tat - tat - tat that ass startin static with dre , make way for the ak that i bring as i slang like cavy 熊熊烈火這晚像夢像幻在燃燒把心燒燙誰令我思緒在搖晃默默流汗是你目光
In trpsin tolerance assay . this virus could resist to 1 % trpsis at 37 in an hour . in acid tolerance assay , this virus was resistant to ph3 . 0 and ph5 . 0 at 37 in 2 hours , and the average infection litre of the virus decreased little . in heat assay , at 50 , the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree . among these conditions , when at 50 in 30 minutes . the average infection litre of this virus decreased over 2 tilre . and when al 50 in an hour , cpe of ihis virus disappeared . when time was set for an hour . but with processed in different temperature as 50 60 70 , 80 , the virus losl the multiplication capacity complelely . in biological assay , we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory . then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second . with f81 cell line , after passaged for 12 times continuously with low concentration of fcs . the virus could produce cpe . however , with vero cell line . the virus could not procuce any cpe after many passages . the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig , fowl and cavy . by neutrolizaion assay , dxmv could be identified as a kind of ccv 理化學(xué)研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經(jīng)37 、 1小時處理的病毒,仍然能夠在貓源細(xì)胞fcwf細(xì)胞上生長,并且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經(jīng)37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恒定溫度50 ,設(shè)定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恒定時間1小時,設(shè)定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細(xì)胞上完全喪失增殖能力, cpe消失。生物學(xué)試驗,利用實驗室現(xiàn)有條件,選擇不同的細(xì)胞系對該病毒進(jìn)行培養(yǎng),發(fā)現(xiàn)該病毒對貓源細(xì)胞fcwf最敏感; mdck細(xì)胞次之; f81細(xì)胞經(jīng)多次傳代,亦可出現(xiàn)cpe ;而vero細(xì)胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細(xì)胞均無血凝性。