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1. And maxi chimerism rate is 6 % estimated from fish results of 32 bacs
   對(duì)32個(gè)bac克隆的熒光原位雜交結(jié)果表明，文庫(kù)的最大嵌合率為6 。
2. Hematopoietic chimerism produces durable and robust immune tolerance to kidney allografts , although incomplete tolerance to donor skin grafting
   造血嵌合能夠?qū)δI移植產(chǎn)生持久有力的免疫耐受，但對(duì)于皮膚移植無(wú)效。
3. These data showed that the chimerism were specially tolerant to donor xenoantigens in skin grafting and mixed lymphocyte reaction ( mlr ) assays , however were still immunocompetent to mhc - disparate third party c57bl / 6 mouse . in the second experiment , mice of group b appeared with variant degree of wasting , diarrhea , fur losing , and all died within 15 days . pathologic changes of liver and intestine were inflamming cells infiltrating and necrotizing
   肝、小腸切片均有明顯病理改變，主要表現(xiàn)為腸粘膜部分脫落、壞死和炎性細(xì)胞浸潤(rùn)，肝實(shí)質(zhì)內(nèi)有灶性淋巴細(xì)胞及多形細(xì)胞浸潤(rùn)，并可見(jiàn)局灶性壞死，兩者均符合gvhd病理改變。
4. Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras . the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02 , has enough polymerism and good stability , and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line , especially when involving strains that can not be discerned with coat color or biochemical markers
   嘗試應(yīng)用多位點(diǎn)dna指紋技術(shù)，檢測(cè)經(jīng)過(guò)胚胎干細(xì)胞es細(xì)胞途徑所獲得的嵌合體小鼠中es細(xì)胞在各種臟器中的嵌合情況檢測(cè)es細(xì)胞在嵌合體小鼠中是否實(shí)現(xiàn)種系傳遞。結(jié)果表明：采用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點(diǎn)dna指紋圖譜，具有足夠的多態(tài)性和很好的穩(wěn)定性。
5. This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability , then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection . finally , first chimeric pig in china was delivered , and by chimera analysis , chimerism is present in some tissues
   本研究首先利用近交系昆明白小鼠進(jìn)行胚胎顯微注射觀察發(fā)育效果，然后利用所獲五指山豬eg細(xì)胞制作嵌合體檢驗(yàn)eg細(xì)胞多能性，最終獲得國(guó)內(nèi)首例胚胎干細(xì)胞( eg細(xì)胞)嵌合體豬，經(jīng)dna分析確認(rèn)在多種組織中存在嵌合狀態(tài)。