the combinant bait plasmid pgbkt7-acam2 was constructed 構(gòu)建了誘餌蛋白表達(dá)載體pgbkt7-acam2。
the holder of the patent thereof enjoys an exclusive right on the inventions, i . e . the re-combinant genetic information through the control over the material carrier thereof and the related acts 斬u制帆影響的只是生物材進(jìn)步帶來的利益分酉d各局斯u益分規(guī)制問題。
the gpa1 gene was obtained via pcr amplification and was cloned in the two-hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7-ga .-galactosidase assay indicated that got did not have the property of self-activation . after pgbkt7-ga was transformed to yeast pj69-4a, we transformed arabidopsis vegetative tissue two-hybrid cdna library plasmids to yeast pj69-4a containing pgbkt7-ga 通過pcr擴增得到gpa1基因并將其克隆到雙雜交dna結(jié)合域載體pgbkt7中,得到的重組質(zhì)粒命名為pgbkt7-g,?半乳糖苷酶活性鑒定表明g不具有自激活特性,將其轉(zhuǎn)化到酵母菌pj69-4a中,再以此為受體菌轉(zhuǎn)化擬南芥綠色營養(yǎng)組織cdna文庫質(zhì)粒。