In this paper , we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid 以dig - oligo2為探針,菌落原位雜交篩選cosmid文庫,得到56個陽性重組子。
The me2155 genomic library was constructed using phz132 as the cosmid vector , and 10 positive clones were fished out using 0 . 9kb pcr product as probe 以phz132為載體,構(gòu)建了mc ~ 2155的基因組文庫。以0 . 9kb的擴(kuò)增產(chǎn)物為探針從文庫中釣出10個陽性克隆子。
Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites 為了獲得膽固醇氧化酶基因,以大腸桿菌-鏈霉菌雙功能柯斯質(zhì)粒phz1357為載體,構(gòu)建了灰色鏈霉菌atcc14811的基因組文庫。
The two end - points were localized on a 4 . 3kb bamhl - ecor1 fragment of a cosmid 16c3 and a 1 . 2kb sail fragment of a cosmid 17g7 respectively . the deletion junction was localized on a 2 . 8kb bamhi fragment of the zx1 chromosome 兩個端點(diǎn)分別定位在野生型菌株基因組文庫粘粒16c3的4 . 3kb的bamh1 - ecor1片段上和17g7的1 . 2kb的sal片段上,缺失界點(diǎn)定位在zx1染色體上的一個2 . 8kbbamh片段上。
Then , we find out the write clones through the chemis hib . during the working , three probes which we used are from the published sequences of spidroin . after this , a genie library of subclone of dragline was constructed 然后我們從構(gòu)建蛛絲蛋白的亞克隆文庫著手,采用鳥槍法測序的策略,取從蜘蛛cosmid文庫中經(jīng)過證實(shí)的陽性克隆scos - ds1 ,大,小為40kb左右,用cai , eco47 ,和hinf三種酶分別對其進(jìn)行酶解分析。
cosmidとは意味:{名} : コスミッド cosmid meaning: Noun: cosmid (genetics) a large vector that is made from a bacteriophage and used to clone genes or gene fragments Type of: vector Encyclopedia: Cosmid cosmid ?:????cosmid перевод:космида (гибридная векторная ДНК)