Rt - pcr kit was from takara technology co . ltd . the sequence of the primer of pkb are shown below , together with all the sodn and saodn oligodeoxynucleotide were synthesized in takara technology co . ltd . pkb primers ; forward primer 5 ' cga gaa gcc gcg acc caa cac3 ' and reverse primer 5atg cac tac cgc cgg a3 " 有絲分裂前, cdcz / cy山b 、組蛋白h ;激酶的活性通過被weel和myt激酶磷酸化cdcz的1卜巧和thr14殘基而抑制。在有絲分裂期mpf可以被cdc25活化,這兩個位點被去磷酸化, cdc25是蛋白水解酶,可以使cdcz的t打巧脫離磷酸化。