例句與用法

1. In the first part , both behavioral responses and fos expression in dorsal horn induced by peripherally injected formalin were observed . animals were divided into three groups ( n = 5 ) : one of them was control with injection of vehicle ( hepes ) into the plantar aspect of the left hindpaw ; and two of them were experimental groups with injection of selective mglur , and mglur5 antagonists , cpccoet or mpep , respectively
   實(shí)驗(yàn)組動(dòng)物山西醫(yī)科大學(xué)的一側(cè)后腳掌分別注入20仍級(jí)碩士學(xué)位論文mg1ur 、 、 mglur 、的拮抗劑cpccoet ( 1oon二01 ) / m尸ep ( 50nmol ) ，對(duì)照組則在一側(cè)腳掌注入緩沖液hpeps (兩種拮抗劑均用hpeps溶解) 。
2. The eg cell culture media consisting of dmem medium supplemented with fbs , chicken serum , beta - mercaptoethanol , l - glutamine . hepes , chicken embryonic extract and cytokines etc . after 24 hours culture , the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
   加入新鮮的eg細(xì)胞培養(yǎng)液(含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細(xì)胞因子等成分)培養(yǎng)24小時(shí)以后， pgcs開(kāi)始部分貼附于共培養(yǎng)的生殖原基細(xì)胞上。
3. Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e . metck darmstadt methods superovulation and collection of eggs for superovulation , female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ) , and after 46 - 48 hours with human chorionic ginadotropin . ( hcg ) . one - cell fertilized eggs were collected on the next day from oviduct of females
   取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg (孕馬血清促性腺激素) 10iu ， 46一48小時(shí)后腹腔注射hcg (人絨毛膜促性腺激素) 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養(yǎng)液中，解剖鏡下撕開(kāi)壺腹，釋放細(xì)胞團(tuán)，然后用300林歲nil透明質(zhì)酸酶消化去除顆粒細(xì)胞，口控吸管將卵細(xì)胞在m :中反復(fù)清洗，然后置于孵箱中，根據(jù)時(shí)間點(diǎn)收集g2期細(xì)胞。
4. After breast feeding , their descendents were fed with the same water , then killed them on the 1st , 8th , 15th , 22nd , 30th day after birth respectively and took out of the brains , stored at - 70 . ( 2 ) measurement of the activity of pkc in brains : in cytoplasma ; took out the samples , cut the hippocampi into pieces and added buffer ( glucose 0 . 32mol / l , hepes ph 7 . 5 ) , then shattered with ultrasound , centri - fuged at 27000g for ih at 4ti , moved the supernant into a new tube
   本研究以細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)途徑為線索，分別探討了pkc 、 erk 、 nos1等信號(hào)分子及即早基因( c - fos ， c - jun )在慢性鉛暴露小鼠腦細(xì)胞中的活性、蛋白表達(dá)及mrna表達(dá)的改變，以及鉛暴露小鼠記憶行為的變化，并將細(xì)胞中信號(hào)分子的改變與記憶功能改變結(jié)合進(jìn)行分析以尋找一些內(nèi)在規(guī)律。