例句與用法

1. quantitative rt-pcr assay involves lightcycler ? technology
   切應力作用時間分別為1h和2h。
2. experimental protocol were performed by instruction manual of lightcycler-rna amplification kit sybr green i . human named genes hgec-40s were used for differential expression screening
   以未作任何處理的內皮細胞作為對照。內皮細胞切應力加載完成后，提取細胞總rna。
3. objective to investigate the effects of fluid shear stress on il-8 gene in human umbilical vein endothelial cells ( huvecs ) and the roles of time course of low shear stress and intensity of fluid shear stress using lightcycler ? system; to investigate the gene expression profiles in huvecs exposed on low shear stress ( 4.20 dyne / cm2, 2 h ) and incubated by 17 p-estradiol ( 10-7 m ) + low shear stress ( 4.20 dyne / cm2, 2 h ) using the cdna microarray approach . methods endothelial cells were isolated from human umbilical cord veins by collagenase treatment as described by jaffe and modified
   目的探討人臍靜脈內皮細胞(humanumbilicalveinendothelialcells，huvecs)在流體切應力作用下il-8基因的誘導表達以及切應力的作用時間和作用強度對il-8基因表達影響的變化規(guī)律；利用表達譜基因芯片技術研究內皮細胞在低切應力(4.20dynecm~2，2h)作用下其切應力相關基因的表達情況以及生理濃度(10~(-7)的17-雌二醇孵育內皮細胞48h，再經同樣條件的切應力處理后對內皮細胞切應力相關基因表達的可能影響。