57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane . 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching . the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared 二是通過原位酶解,抽提蛋白質(zhì)片段進行maldi - tof質(zhì)譜分析,利用肽質(zhì)量指紋圖數(shù)據(jù)在數(shù)據(jù)庫中進行檢索,在雙向電泳凝膠上取了57個點進行分析,有34個蛋白質(zhì)點在數(shù)據(jù)庫得到歸屬鑒定。