Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells , ros and the membrane potential of mitochondria during this process were measured . our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells , led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential , and caused senescence of 2bs cells rapidly . it supports the hypothesis of oxidative damage of senescence . moreover it is a better system for the study of the effects of ros during the process of replicative senescence 利用光學(xué)顯微鏡觀察和酸性-半乳糖苷酶染色技術(shù)研究了高濃度葡萄糖對人二倍體成纖維細胞2bs細胞衰老進程的影響,并用流式細胞儀檢測了此過程中活性氧和線粒體膜電位差的變化。結(jié)果表明: 200 mmol l的葡萄糖對2bs細胞有生長抑制作用,能引起活性氧含量的變化,導(dǎo)致線粒體膜電位差顯著下降,并誘導(dǎo)了細胞的衰老。這為氧化損傷假說提供了新的證據(jù),并為研究活性氧和復(fù)制衰老之間的關(guān)系提供了較好的體系。
microtechniqueとは意味:{名} : マイクロ技術(shù){ぎじゅつ} microtechnique meaning:[Medicine] also mi·cro·tech·nic n : any of various methods of handling and preparing material for microscopic observation and study