例句與用法

1. Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting
   培養(yǎng)多拷貝插入的酵母細(xì)胞，用甲醇誘導(dǎo)目的蛋白的分泌， sds - page和westernblotting鑒定表達(dá)蛋白。
2. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies
   重組質(zhì)粒轉(zhuǎn)化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導(dǎo)多拷貝插入的單克隆酵母細(xì)胞分泌目的蛋白，培養(yǎng)液上清經(jīng)sds - page電泳分析，在蛋白質(zhì)印跡中檢測(cè)到培養(yǎng)液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發(fā)生發(fā)應(yīng)的條帶。
3. Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
   本文將糞產(chǎn)堿桿菌青霉素g?；? afpga )基因構(gòu)建表達(dá)載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動(dòng)子、青霉素g?；富?、 rrnb轉(zhuǎn)錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復(fù)制子；而psmlfpga含有四環(huán)素抗性基因和p15a中拷貝復(fù)制子。
4. The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
   將帶有hwtx -基因的ppic9k經(jīng)blgii線性化后，轉(zhuǎn)化酵母宿主菌gs115原生質(zhì)體后經(jīng)篩選陽(yáng)性克隆并經(jīng)表型鑒定為his ~ + mut ~ s酵母菌，進(jìn)一步用遺傳毒素g418篩選多拷貝的轉(zhuǎn)化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導(dǎo)表達(dá)，發(fā)酵上清經(jīng)90飽和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超濾膜超濾，再經(jīng)cm陽(yáng)離子交換， c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經(jīng)質(zhì)譜鑒定，氨基酸組成分析和序列測(cè)定為正確的表達(dá)產(chǎn)物，生物學(xué)活性表明其活性為天然毒素活性70 % ，表達(dá)量為80mg / l 。