Based on the host reaction and cluster analysis of disease severity , the 57 isolates of venturia inaequalis could be divided into three categories : strong ( pathotype ) , intermediate ( pathotype ) and weak pathogenicity ( pathotype ) respectively 根據(jù)寄主對病菌的反應類型并結合病害嚴重度的聚類分析結果,可將57株蘋果黑星病菌菌株劃分為3個類群:強致病力型、中等致病力型、弱致病力型。
The rest mutations in pp38 and pp24 are at random . sequence analysis also shows the first 195 nuclear acids of pp38 and pp24 are the same except for the 81 site ( g / c ) , but this mutation does not cause the change of amino acid . we regard this as a genetic marker connecting with geography in the evolution of mdv but not related to isolated time and pathotype of different strain of mdv i 對pp24基因和pp38基因進行同源性比較分析的結果表明,絕大多數(shù)毒株二者的前195個核苷酸完全一致,不同毒株間的第81位核苷酸的差異( g / c )并不引起編碼的氨基酸變化,僅僅與地域分布有關,這很可能是mdv在長期病毒衍化過程中形成的地域性遺傳標志,而與病毒的分離年代及mdv的致病型等因素無關。
But the primers designed for vvibdv can only amplify vvibdv strains . so we can typing strains between vvibdv and cibdv by using vvibdv - specific primers by one reaction . typing the pathotype of field ibdv in guangxi , 23 field sample " fragments were amplified by the primary rt - pcr or nested - pcr . they were cleaved with sspl and sacl 第三,應用本研究建立的rea和vvibdv型特異性rtpcr技術,對34份采自廣西不同地區(qū)的ibdv病料,其中pcr反應陽性的23份的pcr產物,進行酶切分型和特異性引物擴增。