Always use automatic pipettes or pipetting devices . no mouth pipetting is permitted 必須使用自動(dòng)吸移管或吸移工具,不可使用口用吸移管。
To make pipetting easier , we will use an enzyme master mix that already contains the appropriate buffers and the two restriction enzymes 為了能在吸取液體時(shí)操作方便,我們會(huì)利用已經(jīng)含有適當(dāng)緩沖溶液與兩種限制酶的酵素反應(yīng)混合液來操作。