the condensation of aromatic aldehyde with barbituric acid, 2-thiobarbituric acid in water 硫代巴比妥酸的縮合反應(yīng)
according to their pathogenic activity, three strain of marine bacteria were selected as l2 ( alteromonas sp ), g ( pseudomonas sp ), py ( pseudomonas sp ) . the biochemical changes of malondialdehyde ( mda ), which is the lipid peroxidation end product and can be measured by thiobarbituric acid ( tba ) regeant, and polyphenol content, which reflected the host non-specific chemical defense activity and can be measured by folin-ciocalteu method, were determined at different time intervals during host pathogenesis 本研究采用海帶作為模式藻類(lèi)生物,以褐藻酸降解菌l2,g及非褐藻酸降解菌py為復(fù)染菌建立人工復(fù)染體系,用硫代巴比妥酸(tba)試劑測(cè)定抗感染過(guò)程中海帶組織細(xì)胞膜脂肪氧化產(chǎn)物丙二醛(mda)的變化情況及folin-ciocalteu酚試劑測(cè)定病原菌感染過(guò)程中海帶組織中多酚含量隨時(shí)間變化情況。