tocs throttle opener control system 節(jié)氣門(mén)強(qiáng)制開(kāi)啟控制系統(tǒng)
the ubi-sl-tocs fragment was taken out, inserted into the multi-cloning site of pcambia1300 vector, transformed into jm109 strain finally, positive colonies were screened on lb plate ( 60 g / ml kan added ) . the result of pcr and enzyme digestion of plasmid proved that recombinatin vector was obtained ( named pcusaib4 and pcusaibu ) 把擴(kuò)增產(chǎn)物分別通過(guò)clai和bamhi酶切純化,連接到用clai和bamhi切去gfpml基因的中間表達(dá)載體pugfpocs中,轉(zhuǎn)化大腸桿菌jm109,在含amp(100g/ml)的lb抗性平板上篩選到了的陽(yáng)性菌落。