Prior to the vector construction , the availability of gene replacement was extensively tested in streptomyces . sp . fr - 008 在載體構(gòu)建之前,我們先在鏈霉菌fr - 008中驗(yàn)證了基因置換方法的可行性。
Such deficiencies cause lysosomal storage disorders , such as gaucher ' s disease , and replacement of the gene is an effective treatment 這樣的缺陷引起溶酶體堆積?。ɡ?,高歇?。?,基因置換是一種有效的療法。
2kb or 7 . 2kb regions abolished the productivity of antibiotic fr - 008 . the expected strain after gene replacement in the region of 5 . 2kb have not been obtained for unknown reason 盡管由于未知的原因尚未獲得5 . 2kb區(qū)域的基因置換菌株,但是以上的試驗(yàn)證明基因置換過(guò)程是可以在鏈霉菌fr - 008中進(jìn)行的。
Phz621 - phz622 , the new gene replacement vector system , had been constructed through the insertion of the 1 . 0kb and 1 . 4kb flanking sequences of hau3r gene from wild - type s . lividans in the same natural orientation 預(yù)期在攜帶有大插入片段的基因置換yac分子進(jìn)入野生型變鉛青鏈霉菌后, yac分子將通過(guò)1 . 4kb或1
Lividans mutant strains la1 and la2 generated by gene replacement in dndb and dndc were obtained respectively . both of these two mutants lost dnd phenotype because of disruption of a respective dnd gene 首先利用基因置換實(shí)驗(yàn)獲得分別在dndb 、 dndc基因內(nèi)部發(fā)生基因中斷的突變菌株la1利la2 ,并通過(guò)southern雜交進(jìn)行了驗(yàn)證。