Role of the hepatocellular microsome cytochrome p450 of rats in regulation of body temperature 450在體溫調(diào)節(jié)中作用的探討
Preliminary data indicate that steviol may be activated to a mutagenic metabloite by human liver microsomes 初步數(shù)據(jù)顯示,甜菊醇可能經(jīng)人體的肝臟微粒體活化成引致基因突變的代謝物。
German standard methods for the examination of water , waste water and sludge - sub - animal testing group t - part 4 : determination of the genotoxic potential using the salmonella microsome test ames test 德國(guó)對(duì)水廢水和污泥的標(biāo)準(zhǔn)檢驗(yàn)法.亞門(mén)動(dòng)物試驗(yàn)方法
While some data suggest that epoxidation may be involved in the metabolic activation of steviol , other data indicate that the active metabolite is not an epoxide . preliminary data indicate that steviol may be activated to a mutagenic metabloite by human liver microsomes 有些數(shù)據(jù)指出,甜菊醇在代謝活化的過(guò)程中,可能牽涉環(huán)氧化作用,但其他數(shù)據(jù)顯示,甜菊醇的活性代謝物并不是環(huán)氧化物。
Now transgenic cell lines that express the protein encoded by ugt gene were broadly used instead of liver microsomes . the enzyme expressed in cell lines helps to realize the function of a specific gene and will provide direct information related to the isozyme interested ‘為解決上述問(wèn)題,國(guó)際上人們己經(jīng)廣泛采用建立各種ugt轉(zhuǎn)基因細(xì)胞系的方式試驗(yàn),在明確基因構(gòu)成的倩況下直接獲得由特定基因所表達(dá)的ugt同工酶, fi什對(duì)性地研究該酶對(duì)藥物的代謝作用。
Knowledge of distribution and regulation of ugts remains limited . liver microsomes is a frequently used enzyme source in in vitro study of glucuronidation , but it has the following deficient respects : 1 . liver microsome is a mixture of various enzymes , and therefore the results from microsome cannot represent the function of a individual isozyme 關(guān)于ugts的組織分布和調(diào)節(jié)這方面的知識(shí)還不多,過(guò)去常采用肝微粒體進(jìn)行研究,但有以下缺陷: u )肝微粒體是多種藥酶沮合的復(fù)雜體系,因而實(shí)驗(yàn)結(jié)果難以區(qū)分是一種同工醇的作用
Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus . for higher plants , however , though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes , only limited evidence is available for the presence and properties of the ip3r - like protein so far . here , using the immunological methods with an antibody raised against a mammalian inositol 1 , 4 , 5 - triphophate receptor ( type 1 ) , we found that , 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively 本實(shí)驗(yàn)用sds - page電泳和免疫印跡的方法,用哺乳動(dòng)物大鼠三磷酸肌醇受體的多肽做抗體對(duì)類三磷酸肌醇受體蛋白鑒定,結(jié)果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應(yīng),同時(shí)還發(fā)現(xiàn)在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應(yīng)的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發(fā)現(xiàn)類三磷酸肌醇受體蛋白主要分布于液泡膜和細(xì)胞質(zhì)膜上。
Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure 擬南芥表皮條用甲基紫精處理,可以引起氣孔的關(guān)閉,當(dāng)處理90分鐘的時(shí)候,氣孔的相對(duì)開(kāi)度由100降到58 ,統(tǒng)計(jì)學(xué)分析氣孔開(kāi)度的變化有顯著差異;甲基紫精引起氣孔關(guān)閉的作用可以被肝素部分抑制,當(dāng)甲基紫精和肝素同時(shí)處理90分鐘的時(shí)候,氣孔的相對(duì)開(kāi)度由100降到92 ,統(tǒng)計(jì)學(xué)分析氣孔開(kāi)度的變化沒(méi)有顯著差異,說(shuō)明氣孔的相對(duì)開(kāi)度基本上沒(méi)有變化。
This experiment was performed using the " yingqing " peach fruit and " chunxing " strawberry to study the changes of the main physiology - biochemistry characteristics and the microsome membrane ca + - atpase activities during the fruits ripening . the effects of ethylene , trifluoperizine ( tfp ) + ethylene and verapamil ( ver ) + ethylene treatments on the above changes were discussed . the objects of the thesis may further explore the role of calcium messenger in the ethylene signal transduction , and provide some new theoretical basis for further research of the microsome membrane ca2 + - atpase during the fruits ripening 本論文以迎慶桃和春星草莓為試材,研究了兩類果實(shí)采后在25和4條件下主要生理生化變化和ca ~ ( 2 + ) - atpase活性變化以及乙烯、鈣調(diào)素拮抗劑tfp +乙烯和鈣通道阻塞劑ver +乙烯處理對(duì)其的影響,為探討果實(shí)成熟衰老過(guò)程中,鈣信使系統(tǒng)在乙烯信號(hào)轉(zhuǎn)導(dǎo)中的作用,提供一定的理論依據(jù),并為探索ca ~ ( 2 + ) - atpase在果實(shí)衰老中的作用及作用機(jī)制,提供實(shí)驗(yàn)證據(jù)。