cleavaged造句
例句與造句
- angiostatin ( as ) is a kind of polypeptide identified as a product of plasminogen cleavaged by protease . it blocks neovascularization by directly inhibiting endothelial cell proliferation and migration
血管抑素(angiostatin，as)是血漿纖溶酶原(plasminogen，plg)經(jīng)蛋白水解酶降解生成的多肽片段，是直接作用于血管內(nèi)皮細(xì)胞的血管生成抑制劑。 - the sds-page results showed the fusion protein was efficiently expressed in the soluble form . 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli . k12
三、對(duì)以上表達(dá)的融合蛋白進(jìn)行初步純化以及利用enterokinase切割融合蛋白，并進(jìn)行抗菌活性檢測(cè)，結(jié)果表明所設(shè)計(jì)的cmiv突變體具有抗菌活性。 - in this study, the recombinant plasmid pmd-18t-pea-h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet-28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet-28c-pea-h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli, the transformed hosts were induced by iptg, bysds-page and elisa analysis of host protein . the expression of the objective gene was detected, and it could account for 16.28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg
同時(shí)將原核表達(dá)載體pet-28c用nco，xho雙酶切，回收酶切產(chǎn)物，將回收的酶切產(chǎn)物pea，h3，載體進(jìn)行連接，并轉(zhuǎn)入dh5感受態(tài)細(xì)胞內(nèi)，培養(yǎng)12-18小時(shí)后，挑取陽(yáng)性菌落，經(jīng)nco，xho雙酶切分析及pcr檢測(cè)，篩選到陽(yáng)性克隆，其質(zhì)粒測(cè)序結(jié)果表明成功地構(gòu)建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達(dá)載體。 - It's difficult to find cleavaged in a sentence. 用cleavaged造句挺難的