例句與造句

1. the recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer . after being packed by lipofectaminetm2000, the recombinant was transfected into b lymphocytes . cd40 expression on membrane, cell proliferation and antibodies concentration were detected with flowcytometry, mtt colorimety assay and el1sa, respectively
   以脂質(zhì)體為介質(zhì)瞬時(shí)轉(zhuǎn)染健康人及sle患者b細(xì)胞系，利用流式細(xì)胞技術(shù)檢測(cè)膜cd40的表達(dá)情況，并利用mtt比色法檢測(cè)細(xì)胞的增殖能力，elisa法檢測(cè)培養(yǎng)液的ig濃度，以研究b細(xì)胞在cd40被抑制以后增殖能力、抗體分泌的改變。
2. mdr1 express product p-glycoprotein was detected by immunocytochemical method and flowcytometry . the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf-7 and mcf-7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine . the pgp expression of mcf-7 adr was strongly positive, the positive rate was 15 %; the pgp expression of mcf-7 was negative, the positive rate was 1.8 % . ic50 of tea polyphenol to mcf-7 and mcf-7 adr is 115.2g ml and 207.6g ml respectively . ic50 of quinidine to mcf-7 and mcf-7 adr is 129.8mol l 42.1g ml and 94.1mol l 30.5g ml respectively . tea polyphenol and quinidine changed little toxicity of adriamycin to mcf-7, but tea polyphenol and quinidine improved the sensitivity of mcf-7adr to adriamycin significantly . immunocytochemistry and flow cytometry can detect p-glycoprotein expression level qualitatively and quantitatively . tea polyphenol is not only an anti-tumor agent, but also a multidrug resistant modulator similar as quinidine . tea polyphenol is advantageous for its little toxicity in tumor treatment
   用免疫組化法和流式細(xì)胞儀對(duì)腫瘤細(xì)胞系mcf-7和mcf-7adr的p-糖蛋白表達(dá)水平進(jìn)行定性定量研究。用噻唑藍(lán)比色法mtt研究茶多酚的細(xì)胞毒性及其對(duì)耐藥性的逆轉(zhuǎn)作用，并與pgp抑制劑奎尼定進(jìn)行了比較。免疫組化法檢測(cè)p-糖蛋白表達(dá)水平，mcf-7adr呈強(qiáng)陽性，而mcf-7呈陰性流式細(xì)胞儀定量檢測(cè)結(jié)果mcf-7adr細(xì)胞系細(xì)胞陽性率為15%，mcf-7細(xì)胞系細(xì)胞陽性率為1.8%。
3. It's difficult to find flowcytometry in a sentence. 用flowcytometry造句挺難的