例句與造句

1. Each segment of dna thus synthesized is called a replicon .
   這樣合成的每一片段DNA稱為復(fù)制子。
2. Each segment of dna thus synthesized is called a replicon
   這樣合成的每一片段dna稱為復(fù)制子。
3. For vector pbmb1205 and pbmb1205r , the plasmid original replicon ori44 was come from b . thuringiensis subsp
   它們的構(gòu)建為今后構(gòu)建高毒力基因工程菌提供了很好的材料。
4. Kurstaki strain hd263 . the plasmid original replicon ori1030 of vector pbmb1206 and pbmb1206r was cloned from b . thuringiensis subsp . kurstaki strain hd73
   該類載體的構(gòu)建在國內(nèi)尚無人報道，國外也沒有成熟的實用性好的解離載體的報道。
5. Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors , pbmb1205 , pbmb1205r , pbmb1206 and pbmb1206r . there are multiple clone sites between two copies of res sites
   解離載體的構(gòu)建和性能利用來源于不同質(zhì)粒上的質(zhì)粒復(fù)制起始區(qū)ori44和ori1030構(gòu)建了4個解離載體。
6. It's difficult to find replicon in a sentence. 用replicon造句挺難的
7. Therefore , the 4 . 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm , ) and resulted in a cloned strain bl - 3 . this strain could express only 130kda protein
   它不僅對蘇云金芽胞桿菌工程菌的構(gòu)建具有現(xiàn)實意義，而且對其它基因高表達研究將產(chǎn)生積極影響，具有一定的理論意義和潛在的應(yīng)用價值
8. A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
   進一步序列分析表明該復(fù)制區(qū)至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復(fù)制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復(fù)制相關(guān)蛋白無同源性。 30連續(xù)培養(yǎng)72h ，復(fù)制區(qū)質(zhì)粒在bt無晶體突變株hd73cry ~ -中穩(wěn)定性達98以上， 30h生長曲線也表明該復(fù)制區(qū)能夠在bt中穩(wěn)定復(fù)制和遺傳，對受體菌株無明顯不良影響。
9. Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
   本文將糞產(chǎn)堿桿菌青霉素g?；? afpga )基因構(gòu)建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g?；富?、 rrnb轉(zhuǎn)錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復(fù)制子；而psmlfpga含有四環(huán)素抗性基因和p15a中拷貝復(fù)制子。