transformant造句
例句與造句
- The fermentation liquid of the transformant
轉(zhuǎn)化子發(fā)酵液也具有脫毛效果。 - In contrast , most of the pollen grains in the non - transformant have plump shape and were stained into red color uniformly
轉(zhuǎn)正向表達(dá)載體在煙草正常花粉粒的百分比較對照植株降低了42 。 - The transformant would flower in 8 to 10 weeks , sometimes under in vitro culture , after the leave dies was infected by agrobacterium tumefaciens
轉(zhuǎn)化植株從浸染起8 - 10周可以開花，甚至在培養(yǎng)瓶中即可完成其生活史。 - And another change was that the spore of the transformant was difficult to release , p3a was not useful in genetically engineered strain construction in this way
此外， pcr擴增發(fā)現(xiàn)轉(zhuǎn)化子比出發(fā)菌ybt - 1520多了cry1ab的特異帶， sds - page電泳證實cry1ab的存在。 - Confirmation of transformant . integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification
通過pcr擴增表明，轉(zhuǎn)基因植株中可以檢測出有目的基因( ipt基因)的存在，目的基因已成功地插入菊花的基因組中。 - It's difficult to find transformant in a sentence. 用transformant造句挺難的
- Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
我們提取了km71ppic9k - fl轉(zhuǎn)化菌株的基因組dna進(jìn)行southern實驗，檢測目的基因的整和插入；提取總rna ，進(jìn)行了northern實驗，檢測fl基因在轉(zhuǎn)化菌株中的表達(dá)。 - Transformant ybt - 833 - 1 kept all indigenous plasmids , ybt - 833 - 2 lost a plasmid carrying crylab and ybt - 833 - 3 lost all indigenous plasmids carrying icp genes . comparing their potency with natural strain ybt - 833 , the overall toxicity of all transformants was not noticeably higher than strain ybt - 833
質(zhì)粒pbmblc電脈沖轉(zhuǎn)入菌株ybt - 833 ，得到了幾個icp基因背景不同的轉(zhuǎn)化子，它們的綜合毒力較出發(fā)菌ybt - 833都沒能得到提高。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經(jīng)blgii線性化后，轉(zhuǎn)化酵母宿主菌gs115原生質(zhì)體后經(jīng)篩選陽性克隆并經(jīng)表型鑒定為his ~ + mut ~ s酵母菌，進(jìn)一步用遺傳毒素g418篩選多拷貝的轉(zhuǎn)化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導(dǎo)表達(dá)，發(fā)酵上清經(jīng)90飽和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超濾膜超濾，再經(jīng)cm陽離子交換， c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經(jīng)質(zhì)譜鑒定，氨基酸組成分析和序列測定為正確的表達(dá)產(chǎn)物，生物學(xué)活性表明其活性為天然毒素活性70 % ，表達(dá)量為80mg / l 。 - Plant expression vector lba4404 ( pbin + - 35s - cbf3 + pal4404 ) was used in the genetic transformation of cucumber and micro - tomato . establishment of high efficiency regeneration system and determination of parameters involved in agrobacterium - mediated transformation , screening and characterization of transformant were systemically researched
將植物表達(dá)載體pbin ~ + - 35s - cbf3對黃瓜和小番茄進(jìn)行遺傳轉(zhuǎn)化，建立了黃瓜高頻再生和遺傳轉(zhuǎn)化體系，獲得了一株轉(zhuǎn)基因黃瓜植株，并通過了植物基因組pcr檢測；獲得了兩個具有kan抗性的小番茄再生綠芽。 - T . identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis . both analysis the target bands were observed . so the integration of the cpti gene into mustard genome dna was confirmed . the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
轉(zhuǎn)基因芥菜植株的鑒定取再生苗的葉片提取dna ，進(jìn)行pcr擴增和pcrsouthernblot分析，轉(zhuǎn)化再生植株大部分呈陽性，而非轉(zhuǎn)化的再生植株均為陰性，證明cpt基因已存在于芥菜基因組中。 - 16 replacement plasmids for two different regions were obtained , but have n ' t been introduced into 10 - 22 successfully . 42 cosmids in this region were introduced into a heterogeneous host zx64 , one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton
將300kb區(qū)域的柯斯質(zhì)粒進(jìn)行異源表達(dá)，發(fā)現(xiàn)有一文庫質(zhì)粒5h4的轉(zhuǎn)化子在硫鏈絲菌素存在時表現(xiàn)出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。 - The condition of somatic embryogenesis under different hormone conditions was discussed . the amount of embryo decreased but the amount of calli increased as the increase of kt concentration under the same 2 , 4 - d level after embryogenesis calli were transferred to medium with no hormone . the suitable concentration of kanamycin for selecting transgenic explants in the course of carrot tissue culture was screened in order to strictly select transformant
2 ， 4 ? d濃度相同kt濃度不同的激素組合隨著kt濃度的升高，誘導(dǎo)的胚性愈傷轉(zhuǎn)至不含激素的培養(yǎng)基上后胚狀體發(fā)生量及發(fā)生胚狀體的塊數(shù)都隨kt濃度增加而減少，但愈傷組織塊的生長量卻隨kt的濃度升高而增加。