Expression amount of rhbsag in the former was increased 60 % and 80 % respectively than the latter . antigenicity of pres2 of fusion protein was increased 8 times and 10 times in bmn cells and in pupa hemolymph respectively than nonfusion hbsag Bmpak - hbmp表達的融合m蛋白( pres2 + s )中, pres2抗原性顯著提高,與bmpak - hbm表達的非融合蛋白相比,融合蛋白pres2抗原性在細胞中約提高了8倍,蛹中約提高了1o倍。
The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting . the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized ) 用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原( pres2 + s )基因,為本實驗構(gòu)建]感染家蠶細胞及蛹,對兩種病毒的表達產(chǎn)物用elisa進行了跟蹤檢測,結(jié)果表明,感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3