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  • 例句與用法
  • Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2 , signal molecules , skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ) . radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation . ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method
    應(yīng)用免疫組織化學(xué)方法光鏡下觀察高滲刺激后,大鼠視上核膠質(zhì)細(xì)胞(星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞)受體( nmdar2 ) 、信號(hào)分子、骨架蛋白及縫隙連接蛋白的表達(dá)的時(shí)空變化;應(yīng)用放免測定檢測高滲刺激前后血漿中vp含量。
  • We injected carbennoxolone , a gap junction blocker , into the lateral ventricle , which was followed by hyperosmotic stimulation , the immunohistochemical staining of neurons and astrocytes and vp content in plasm were studied . western blot was performed to detect the content of cx43 and cx32 in son following hyperosmotic stimulation
    側(cè)腦室注射縫隙連接阻斷劑甘珀酸( carbenoxolone , cbx )后,再給予高第四軍醫(yī)大學(xué)博士學(xué)位論文滲刺激,觀察視上核膠質(zhì)細(xì)胞和神經(jīng)元免疫組化反應(yīng)的改變,并檢測血漿vp含量的變化。
  • 3 , vp content in plasma increased significantly 45min after hyperosmotic stimulation . when pre - injected carbennoxolone , a gap junction blocker , into the lateral ventricle , followed by hyperosmotic stimulation , vp content remained the base line . the expression of gfap - li astrocytes showed no difference , while that of fos - li neurons decreased significantly
    門)高滲刺激后扔dn ,血漿中p含量明顯升高,若事先經(jīng)側(cè)腦室注入cbx后再給高滲刺激,血漿中vp含量不升高,視上核內(nèi)星形膠質(zhì)細(xì)胞gfap陽性反應(yīng)與單純高滲組無差別,而fos陽性神經(jīng)元明顯減少。
  • Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz , pvn and son respectively ; ( 2 ) under electronic microscope , the astrocytic processes connected closely with the dendrites or axons of the neurons , where the bilateral membranes became thick . we call transiently it electron - dense areas ( edas ) . the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely , wga - hrp was microinjected into the unilateral son , pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method , the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz , son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution , intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
    腦內(nèi)gfap陽性結(jié)構(gòu)也明顯增多,其分布與fos陽性細(xì)胞分布基本一致,表現(xiàn)為胞體肥大、突起粗長; ast緊密包繞在神經(jīng)元周圍形成神經(jīng)元- ast復(fù)合體( n - asc ) ;在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫(yī)大學(xué)博士學(xué)位論文和fos vp gfap三重標(biāo)記asc ; ( 2 )免疫電鏡下son內(nèi)星型膠質(zhì)細(xì)胞突起與神經(jīng)元樹突或軸突之間接觸部位出現(xiàn)增厚的膜結(jié)構(gòu)一電于致密區(qū)( edas ) ,高滲刺激后數(shù)量明顯增多: ( 3 )將們個(gè)mp注入大鼠一側(cè)n卜、卜卜或孤束核( ws ) ,分別在延髓內(nèi)臟帶( mvz ) 、 so和pvn內(nèi)出現(xiàn)fos hrp th 、 fos hrp八p三重標(biāo)記神經(jīng)元和gfap陽性標(biāo)記ast形成的n asc ; ( 4 )高滲刺激使培養(yǎng)神經(jīng)元和ast內(nèi)鈣水平先升高后降低,最后維持在比高滲刺激前稍高的靜息鈣水平上。
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