The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products . when there were two dna fragments of 363bp and 305bp were produced from a pcr product , the strains were assumed to have a mutation at ser83 , when three fragments of 363bp , 206bp and 99bp were produced , the strains were assumed to have no mutation at the 83or 116 . the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp 本研究重點(diǎn)分析了16株菌對(duì)氟喹諾酮類( fqns )藥物的耐藥性,結(jié)果表明: 16株菌對(duì)諾氟沙星、環(huán)丙沙星、沙拉沙星、單諾沙星和氧氟沙星等( nor 、 cip 、 sar 、 dan 、 ofl )的耐藥率在31 . 3 - 56 . 3之間。
Some characteristics of cold - active protease and chitinase were analyzed then . microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences . the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area , as well as the interaction between microbial community and environment , were analyzed based on these studies 通過(guò)構(gòu)建沉積物中微生物16srdna克隆文庫(kù),采用pcr - rflp分析、 dna - dna雜交、 16srdna序列測(cè)定以及系統(tǒng)發(fā)育分析的方法,研究了兩太平洋“暖池”區(qū)和東太平洋“結(jié)核”區(qū)兩個(gè)不同海區(qū)深海沉積物中的微生物多樣性和群落結(jié)構(gòu)特征及其與環(huán)境的相互關(guān)系,得到了一些與“暖池”區(qū)環(huán)境特點(diǎn)緊密相關(guān)的新發(fā)現(xiàn),新認(rèn)識(shí)。