In the present study , aflp ( amplified fragment length polymorphism ) markers was used to examine population of abies yuanbaoshanensis in order to understand the level of population genetic variation and genetic structure . the result would help to evaluate its evolutionary potentiality and the degree of being endangered and could provide scientific basis for making right protection strategy . high - quality dna was extracted using ctab method from those tender leaves of forty - three fully - developed trees in population abies yuanbaoshanensis 本研究選用一種高效的檢測(cè)遺傳變異的分子標(biāo)記? ? aflp技術(shù)來(lái)分析元寶山冷杉種群的遺傳多樣性,旨在了解該種群在分布區(qū)內(nèi)的遺傳變異水平和遺傳結(jié)構(gòu)情況;研究結(jié)果將有助于更清楚地認(rèn)識(shí)這一瀕危類群的生存潛力和瀕危程度,而且可以為制定何種挽救和保護(hù)措施提供科學(xué)的依據(jù)。
1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out 具體包括以下幾個(gè)部分: 1 、針對(duì)雁形目鳥(niǎo)類異常復(fù)雜的分類狀況及分類上存在的爭(zhēng)議,根據(jù)雁形目鳥(niǎo)類的形態(tài)學(xué)、行為學(xué)、骨骼學(xué)、角蛋白、線粒體與核dna酶切片段長(zhǎng)度多態(tài)、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究,對(duì)雁形目鳥(niǎo)類分類中科、亞科和族的劃分及其相互間的系統(tǒng)發(fā)生關(guān)系進(jìn)行綜述,分析系統(tǒng)學(xué)研究中存在的不足,提出了雁形目鳥(niǎo)類分類中急需解決的問(wèn)題。
All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc ) 所有的樣本通過(guò)聚合酶鏈?zhǔn)椒磻?yīng)?限制性片段長(zhǎng)度多態(tài)性方法( pcr - rflp )對(duì)ahsg基因的第7個(gè)外顯子內(nèi)的sac多態(tài)性位點(diǎn)進(jìn)行基因分型,該多態(tài)性位點(diǎn)為238號(hào)氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變?yōu)榻z氨酸( ser , agc ) 。
The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates 本研究通過(guò)對(duì)豬繁殖與呼吸綜合征病毒( prrsv )國(guó)內(nèi)分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析,系統(tǒng)研究了國(guó)內(nèi)分離毒株的抗原特性和遺傳學(xué)差異。
Analyse the research of distributing of microbe community and the tendency of the change , disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism , to research the law of change that the microbe community have in composting process , we can get effective and rapid information to filtrate the microorganism during composting process , then accelerate the development of compost technology 摘要對(duì)堆肥微生物種群分布及其動(dòng)態(tài)變化的研究進(jìn)行了分析,論述了分子生物技術(shù)中的變性梯度凝膠電泳和末端標(biāo)記限制性片段長(zhǎng)度多態(tài)性的原理和特點(diǎn),以及用于研究堆肥微生物的群落結(jié)構(gòu)演變規(guī)律,為分析和篩選堆肥中的微生物提供更加有效、快速的信息,促進(jìn)堆肥技術(shù)的發(fā)展。
Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province . 2 . the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough 應(yīng)用熔解曲線法和pcr微板核酸雜交- elisa法對(duì)44例接受拉米夫定治療過(guò)程中出現(xiàn)病毒學(xué)反跳時(shí)的血清進(jìn)行ymdd突變株的檢測(cè),并與測(cè)序法和mpcr - rflp法比較它們的敏感性、一致性。
The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products . when there were two dna fragments of 363bp and 305bp were produced from a pcr product , the strains were assumed to have a mutation at ser83 , when three fragments of 363bp , 206bp and 99bp were produced , the strains were assumed to have no mutation at the 83or 116 . the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp 本研究重點(diǎn)分析了16株菌對(duì)氟喹諾酮類( fqns )藥物的耐藥性,結(jié)果表明: 16株菌對(duì)諾氟沙星、環(huán)丙沙星、沙拉沙星、單諾沙星和氧氟沙星等( nor 、 cip 、 sar 、 dan 、 ofl )的耐藥率在31 . 3 - 56 . 3之間。
The dissertation consists of five chapters : in chapter one , the recent progress in molecular approaches in systematic studies of macroalgae e . g . dna extraction , restriction endonuclease fragment length polymorphisms ( rflps ) , random amplified polymorphic dna ( rapd ) , gene sequencing , intersimple sequence jepeats ( issr ) , amplified fragment length polymorphisms ( aflp ) and single strand . conformation polymorphisms ( sscp ) were reviewed 本論文由五部分組成:在第一部分,綜述了大型海藻dna的提取、限制性片段長(zhǎng)度多態(tài)性( rflps ) 、隨機(jī)擴(kuò)增多態(tài)性dna ( rapd ) 、核酸序列分析、擴(kuò)增片段長(zhǎng)度多態(tài)( aflp ) 、單鏈構(gòu)象多態(tài)( sscp )等分子手段在大型海藻系統(tǒng)學(xué)研究中應(yīng)用的一些進(jìn)展。
Some characteristics of cold - active protease and chitinase were analyzed then . microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences . the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area , as well as the interaction between microbial community and environment , were analyzed based on these studies 通過(guò)構(gòu)建沉積物中微生物16srdna克隆文庫(kù),采用pcr - rflp分析、 dna - dna雜交、 16srdna序列測(cè)定以及系統(tǒng)發(fā)育分析的方法,研究了兩太平洋“暖池”區(qū)和東太平洋“結(jié)核”區(qū)兩個(gè)不同海區(qū)深海沉積物中的微生物多樣性和群落結(jié)構(gòu)特征及其與環(huán)境的相互關(guān)系,得到了一些與“暖池”區(qū)環(huán)境特點(diǎn)緊密相關(guān)的新發(fā)現(xiàn),新認(rèn)識(shí)。