Thus , it will help elucidate the development , self tolerance of self autoreactive b lymphocyte repetoire to investigate the generation of naa . researches on the function of naa will help clarify the significance of naa ' s existence . the exploration of biological nature , encoded gene and the difference or relation between naa and pathological autoantibody will shed much light on the pathological mechanism and treament of autoimmune disease 因此,對naa產(chǎn)生機(jī)理的揭示將有助于回答免疫學(xué)b細(xì)胞產(chǎn)生、發(fā)育以及自身耐受的問題;對naa功能的研究有助于進(jìn)一步明確naa對機(jī)體的意義;對naa的特性、編碼基因、與病理性自身抗體界限和關(guān)系的研究可能對自身第四軍醫(yī)大學(xué)碩士學(xué)位論文免疫疾病的發(fā)病機(jī)制和治療提供有益的線索。
It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions , clearing aged cells and metabolic products , regulating immune responses and protecting against infection . in some pathological states such as psoriasis and contact dermatitis , a certain serum level of the antibody could inhibit the progression of the diseases , and is beneficial to the recovery from the diseases . after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies , meanwhile , experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) " , a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability , immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis , and recombinant antibody is under investigating 抗角蛋白自身抗體( akautoab )是naa的重要組成部分,以往實(shí)驗(yàn)通過雜交瘤技術(shù)、免疫親和層析技術(shù)和噬菌體抗體庫技術(shù)分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ,并對akautoab免疫學(xué)特性及在體生理和病理意義進(jìn)行了廣泛的研究,直接或間接地發(fā)現(xiàn)akautoab是機(jī)體正常免疫調(diào)節(jié)網(wǎng)絡(luò)的組成部分,在維護(hù)某些生理狀態(tài)的穩(wěn)定、清理衰老細(xì)胞及代謝產(chǎn)物、調(diào)節(jié)免疫和抗感染等方面起到重要作用;在某些病理情況下(如銀屑病、接觸性皮炎等) ,體內(nèi)akautoab的組分和滴度會發(fā)生變化,而正常水平的akautoab則有利于限制病情的發(fā)展,促進(jìn)損傷的修復(fù)。
No callus occurred from hairy roots on medium lacking 2 , 4 - d . high concentration ( l . 0mg / 1 ) of 2 , 4 - d could induce much more calli , but it obstructed the growth of calli and followed buds differentiation . the result of callus induction showed that the optimum medium for callus induction was ms + 0 . 5mg / l 2 , 4 - d + 0 . 5mg / l 6 - ba and ms + 0 愈傷組織誘導(dǎo)最佳培養(yǎng)基為: ms 05mg l個(gè)d 05mg l ba ,愈傷組織生長的最佳培養(yǎng)基為ms 0ling 12 , 4 d cling 16ea ;愈傷組織能在不含激素的培養(yǎng)基上自發(fā)地分化出不定芽,但分化頻率較低,而6ea對不定芽的分化作用較明顯,配合使用6習(xí)a和naa對芽的誘導(dǎo)及得芽率較好,不定芽誘導(dǎo)的最佳培養(yǎng)基為ms 10mgilnaa 05mg lia培養(yǎng)基。
The regeneration system of tobacco was established . the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2 . 0mg / l 6 - ba and 0 . 3mg / l naa . then the regenerated plants were rooted on ms medium containing 0 . 3mg / l naa 植株再生體系的建立采用無菌苗煙草葉片為外植體,以ms為基本培養(yǎng)基,篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導(dǎo)不定芽的分化,并于ms + naa0 . 3mg l的pa碩士學(xué)位論文v沉了砂“工隊(duì)nk ”主根培養(yǎng)基上生根,獲得完整再生植株。