To study the suitable method for cattle oviduct simple epithelium cells culture , the epithelium cells were isolated by cutting and 0 . 25 % trypsinization , the exponential phase of growth cells vigor and growth velocity was determined by mtt method , the viable count was detected by the rejection experiment of trypanblau 摘要為探討適用于黃牛輸卵管單層上皮細胞的培養(yǎng)方法,采用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞,取對數(shù)生長期細胞進行mtt比色實驗檢測細胞活力和生長速度;臺盼藍排斥試驗檢測活細胞數(shù)。
百科解釋
Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to a cell culture, trypsin breaks down the proteins which enable the cells to adhere to the vessel.