Sox genes encode a group of proteins that carry a dna - binding hmg domain Sox基因含有一個(gè)能與dna特異結(jié)合的高度保守hmg - box基序。
0 software and the plasmid with correct sequences was maxipreped and stored in - 201 ,構(gòu)建帶有ha標(biāo)簽的人wnk4基因coiled一coilz區(qū)域連同附近一個(gè)基序的表達(dá)載體。
Dy330 ( ayggg ) and dy331 ( ayggg ) showed green color after yggg was knocked out . as analysis by bioinformatics , yggg belongs to peptidase _ m48 family which contains hexxh motif that forms a metal binding site 生物信息學(xué)的分析提示: yggg可能是一種金屬蛋白酶,屬于peptidasem48家族,臼?罕醫(yī)夭念傅士行住公式含有he基序。
Dna sequencing of the appa gene showed an open reading frame of 1299 bp . the deduced appa phytase composed of 432 amino acids ( predicted molecular mass , 47 . 06 kd ) also contained the reserved active - site motif rhgxrxp , which is shared by other phytases and acid phosphatases 測序結(jié)果顯示appa基因閱讀框架為1299bp ,編碼432個(gè)氨基酸,編碼產(chǎn)物理論分子量為47 . 06kd ,同時(shí)它也具有其它植酸酶與酸性磷酸酶的活性保守基序rhgxrxp 。
Bioinformatic analysis were performed to prepro - gynostemminl on the base composition , codon preference , amino acid composition , pi value , molecular weight , secondary structure , prosite scan , prediction of protein localization sites . blast thtf arabidopsis thaliana genome , and 3d structure modeling 運(yùn)用生物信息學(xué)軟件對絞股藍(lán)班p前體進(jìn)行堿基組成和密碼子偏倚性、氨基酸組成特征、功能位點(diǎn)、亞細(xì)胞定位、跨膜結(jié)構(gòu)、核酸結(jié)合基序以及空間結(jié)構(gòu)等方面的分析。
Using transfac 4 . 0 and tssg tssh nsite of softbeny , we can see that a tata box lie in - 188bp - 198bp from transcriptional initiation site , there are several api , c / ebp binding motifs near it or in other 5 " flanking region , a c - fos response element is in - 1133bp - 1143bp from transcriptional initiation site 0軟件和softbeny網(wǎng)站上tssg , tssh , nsi ’ fe軟件共同分析,在a ? ibgp轉(zhuǎn)錄起始點(diǎn)上游j到一198區(qū)域存在tata盒,在其附近及其5 ’上游遠(yuǎn)端調(diào)節(jié)區(qū)存在多個(gè)api , c ebp結(jié)合位點(diǎn),并且在轉(zhuǎn)錄起始點(diǎn)上游人到一處存在一個(gè)c fos結(jié)合基序。
In our study , we found that a - 1bgp expression is up - regulated in hepatoma cells treated with gh . it suggests that gh is capable of stimulating the expression of a - 1bgpin hepatoma cells . so a - 1 bgp may play a role in postnatal growth and intermediary metabolism which are major physiological actions of gh 為了了解gh是通過何種途徑調(diào)節(jié)a ibgp表達(dá),我們分析了a ibgp的5 ’端調(diào)控區(qū),臺果發(fā)現(xiàn)了普遍存在的api , c ebp結(jié)合基序,而且還存在著一個(gè)( , fos結(jié)合基序,所以推測gh可能通過誘導(dǎo)c fos , c一表達(dá)增高,結(jié)合于a ibgp調(diào)控區(qū)的特異性dna反應(yīng)元件,促進(jìn)它的表達(dá)。
The ir sequence of mxmybl is most homologous with that of atmyb ( 86 . 9 % ) and somewhat homologous with that of stmyb 1 ( 77 . 0 % ) ; there is very low homology among n - and c - terminal regions outside of the ir regions of all of the mybs ; the protein mxmybl contains a proline - rich region as well as a serine - rich region near the c - terminus , such structure motifs are implicated in transcriptional activation 9 ,與馬鈴薯stmyb的ir序列的同源性達(dá)77 0 ,所有這些nnrb蛋白除了瓜區(qū)具有較高的同源性外,其c端和n端幾乎沒有同源性。 mwyb蛋白的c一端還含有一個(gè)富含脯氨酸區(qū),這樣的結(jié)構(gòu)基序可能具有激活轉(zhuǎn)錄的功能。
Thioredoxins , an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc - , are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda . by its disulfide / dithiol interchange reaction , this protein can transmit the regulatory signals to seleted targets ( enzymes , transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis , dna synthesis , transcription , protein disulfide reduction , protein repair , filamentous phage assembly , cell apoptosis and seeds germinating and so on 該蛋白質(zhì)中含有保守的- cp ( g ) pc -氨基酸活性基序,該基序中的兩個(gè)半胱氨酸殘基可通過巰基二硫鍵的轉(zhuǎn)換實(shí)現(xiàn)其氧化還原狀態(tài)的變化和電子氫的傳遞,對細(xì)胞中與氧化還原相關(guān)的多種生理過程的調(diào)節(jié)起重要作用。通過同許多酶類、蛋白類、細(xì)胞內(nèi)活性因子相藕連, trx能對光合作用、 dna復(fù)制、基因轉(zhuǎn)錄、細(xì)胞凋亡和生長、噬菌體組裝、蛋白質(zhì)的還原和修復(fù)信號傳導(dǎo)等生理過程產(chǎn)生影響和調(diào)節(jié)。