I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids , to create a gene targeting insertion vector designated gfp tnos . after agro - infiltration into transgenic nicotiana benthamiana 16c , progeny were analyzed genetically for phenotypic changes , sirna accumulation , and dna methylation 采用農(nóng)桿菌浸潤法( agro - infiltration )感染轉(zhuǎn)基因本生煙16c ,并對同源基因瞬時表達所引起的植物表型變化、小分子rna的產(chǎn)生、 dna甲基化程度、以及相關(guān)性狀在后代中的遺傳情況進行了檢查。