Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them . the homological cry3aa gene was expressed in escherichia coli . . and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page 生物活性測定結(jié)果表明該菌株對榆藍(lán)葉甲( pyrrhaltaaenescens ( fairmaire ) )和光肩星天牛等鞘翅目昆蟲具有較高的毒力,因此初步確認(rèn)該菌株屬于cry3類; ( 2 )發(fā)現(xiàn)該菌株中編碼毒蛋白的基因位于質(zhì)粒上,并且已經(jīng)成功地克隆到該基因。