Amplified in e . coli xi - blue , the eluted phage in the third rounds was poured onto lb / iptg / xgal plate . we selected randomly 18 clones and amplified them , then confirmed positive clones by elisa 經(jīng)雙抗體(流感病毒的多抗和辣根過氧化物酶標記m13噬菌體抗體)夾心elisa鑒定的陽性克隆有12個,分別將其純化、并進行dna序列分析。
The amplified dna fragments were inserted into pgem - t easy vector and sequenced . the dna fragment sequencing results from the two subspecies were compared to detect whether there was any difference 將pcr擴增產(chǎn)物克隆到pgem - teasy載體,進行dna序列分析,并用生物信息學(xué)方法比較東方田鼠長江亞種與指名亞種之間該序列的差異。
Method : a total of 101 isoniazid - resistant and 43 susceptible strains of mycobacterium tuberculosis were analyzed by pcr and sequence analysis of their katg , inha , ahpc , kasa , oxyr genes 方法對144株結(jié)核分枝桿菌臨床分離株( 101株異煙肼耐藥株及43株異煙肼敏感株) katg , inha , ahpc , kasa及oxyr基因進行pcr擴增及dna序列分析。
Conclusion the mtdna sequencing system using new primers was useful for forensic purpose . for difficult samples , dna typing with this system was especially available and practical , and has been validated in criminal cases 結(jié)論本課題所建立的線粒體dna序列分析體系是一種對法醫(yī)疑難檢材進行鑒別的有效、實用的方法,并且本方法已經(jīng)在實際案件檢驗中得到驗證。
Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia . the pcr products are recombined with vectors of pmd18 - t . more over , the recombinant plasmids are colonged . the dna sequence analysis shows the insert fragment is momp dna 本實驗采用pcr技術(shù),從l _ 2型沙眼衣原體dna中擴增出momp基因,與pmd18 - t載體重組行t a克隆,并進行dna序列分析,證明所克隆基因為mompdna序列。
dna: DNA = 1.deoxyribonucle ...序列: order; alignment; array; suc ...分析: analyse【統(tǒng)計】序列分析。: sequential analysis時序列分析: time series analysis相序列分析: facies sequence analysis序列分析: sequence analysis; sequencing; sequential analysis序列分析器: sequential analyser; sequential analyzer序列分析儀: sequenator; sequencer核酸序列分析: nucleic acid sequence analysis脈沖序列分析: pulse-train analysis毗鄰序列分析: nearest neighbour sequence analysis; nearest neighbor sequence analysis時間序列分析: analysis of time series; time sequential analysis; time series analysis氨基酸序列分析: amino acid sequence analysis氨基酸序列分析器: amino acid sequence analyzer蛋白質(zhì)序列分析: protein sequence analysis核苷酸序列分析: nucleotide sequence analysis金融時間序列分析: font-weight:boldanalysis of financial time series生物序列分析中心: technical university of denmark - center for biological sequence analysis時間序列分析法: time series analysis method緊鄰序列分析,最近鄰序列分析: nearest-neighbour sequence analysis氣相蛋白質(zhì)序列分析儀: gas phase protein sequencer時間序列分析的假定: hypothesis testing in time series analysis脫氧核糖核酸序列分析: dna sequencing液相蛋白質(zhì)序列分析: liquid-phase protein sequencing