Amplified in e . coli xi - blue , the eluted phage in the third rounds was poured onto lb / iptg / xgal plate . we selected randomly 18 clones and amplified them , then confirmed positive clones by elisa 經(jīng)雙抗體(流感病毒的多抗和辣根過(guò)氧化物酶標(biāo)記m13噬菌體抗體)夾心elisa鑒定的陽(yáng)性克隆有12個(gè),分別將其純化、并進(jìn)行dna序列分析。
The amplified dna fragments were inserted into pgem - t easy vector and sequenced . the dna fragment sequencing results from the two subspecies were compared to detect whether there was any difference 將pcr擴(kuò)增產(chǎn)物克隆到pgem - teasy載體,進(jìn)行dna序列分析,并用生物信息學(xué)方法比較東方田鼠長(zhǎng)江亞種與指名亞種之間該序列的差異。
Method : a total of 101 isoniazid - resistant and 43 susceptible strains of mycobacterium tuberculosis were analyzed by pcr and sequence analysis of their katg , inha , ahpc , kasa , oxyr genes 方法對(duì)144株結(jié)核分枝桿菌臨床分離株( 101株異煙肼耐藥株及43株異煙肼敏感株) katg , inha , ahpc , kasa及oxyr基因進(jìn)行pcr擴(kuò)增及dna序列分析。
Conclusion the mtdna sequencing system using new primers was useful for forensic purpose . for difficult samples , dna typing with this system was especially available and practical , and has been validated in criminal cases 結(jié)論本課題所建立的線粒體dna序列分析體系是一種對(duì)法醫(yī)疑難檢材進(jìn)行鑒別的有效、實(shí)用的方法,并且本方法已經(jīng)在實(shí)際案件檢驗(yàn)中得到驗(yàn)證。
Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia . the pcr products are recombined with vectors of pmd18 - t . more over , the recombinant plasmids are colonged . the dna sequence analysis shows the insert fragment is momp dna 本實(shí)驗(yàn)采用pcr技術(shù),從l _ 2型沙眼衣原體dna中擴(kuò)增出momp基因,與pmd18 - t載體重組行t a克隆,并進(jìn)行dna序列分析,證明所克隆基因?yàn)閙ompdna序列。
Based on the structure and function analysis of hirudin , a potent thrombin inhibitor , and some platelet aggregation inhibitors , which contain the recognition sequence argglyasp as their functional motif , two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus . these chimera genes were constructed by pcr and inserted into the expression vector pet21a , the constructs were confirmed by restriction enzyme digestion and dna sequence analysis . these recombinant plasmids were transformed into 經(jīng)限制酶消化和dna序列分析,證明兩種重組質(zhì)粒與設(shè)計(jì)完全一致。由于rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號(hào)肽序列,在iptg誘導(dǎo)下兩種嵌合分子都獲得了分泌表達(dá),表達(dá)產(chǎn)物主要集中在細(xì)胞周質(zhì)空間。
1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out 具體包括以下幾個(gè)部分: 1 、針對(duì)雁形目鳥(niǎo)類異常復(fù)雜的分類狀況及分類上存在的爭(zhēng)議,根據(jù)雁形目鳥(niǎo)類的形態(tài)學(xué)、行為學(xué)、骨骼學(xué)、角蛋白、線粒體與核dna酶切片段長(zhǎng)度多態(tài)、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究,對(duì)雁形目鳥(niǎo)類分類中科、亞科和族的劃分及其相互間的系統(tǒng)發(fā)生關(guān)系進(jìn)行綜述,分析系統(tǒng)學(xué)研究中存在的不足,提出了雁形目鳥(niǎo)類分類中急需解決的問(wèn)題。
Objective : to elucidate the characterization of katg , inha , ahpc , kasa , and oxyr gene mutations in isoniazid - resistant clinical isolates of mycobacterium tuberculosis , and discuss the value of judging the susceptibility of mycobacterium tuberculosis strains to isoniazid by dna sequencing 目的闡明結(jié)核分枝桿菌耐異煙肼臨床分離株katg基因、 inha基因、 ahpc基因、 kasa基因及oxyr基因突變特點(diǎn),探討是否可能通過(guò)dna序列分析來(lái)判斷結(jié)核分枝桿菌對(duì)異煙肼的敏感性。