Construction and packing of replication - defective adenovirus vectors expressing hcv structural proteins will play an important role in research of hcv and vaccination . hcv infection often result in persistent infection 構(gòu)建并包裝表達(dá)hcv結(jié)構(gòu)基因的復(fù)制缺陷型腺病毒載體,在丙型肝炎的防治和hcv的研究中具有重要意義。
3 . using clamp technique , ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig 供肝冷保存時(shí),采用血管夾技術(shù)lamptechnique )經(jīng)門靜脈灌注攜帶融合基因hllctla4dg的重組腺病毒,于術(shù)后3天、 7天能定性檢測到hllctla4ig在受體外劃血卜1 。