When anthers were cultured on ms medium with 2 . 0 mg / l 6 - ba and 0 . 5 mg / l naa , the inducing rate of darslect and toyonaka was as high as 98 % and 85 % respectively 草莓的基因型不同,誘導(dǎo)率不同,達(dá)賽和豐香的誘導(dǎo)率分別為98和85 ,達(dá)賽的誘導(dǎo)率明顯高于豐香。
After 4 times selected culture by ppt , 347 resistant calli were obtained . the ppt resistant calli were transferred to ms medium with 2 . 0 mg / l 6 - ba , 0 . 1 mg / l naa for regeneration 0mg l +蔗糖30g l的選擇培養(yǎng)基中進(jìn)行選擇培養(yǎng),每10天繼代1次,連續(xù)選擇4次,共得到347塊抗性愈傷組織。
86 . 7 % buds surrived on 1 / 2 ms containing 250 mg / 1 km after 28d . two of six buds were dead 40d after that were put on 1 / 2ms medium containing 0 . 1 mg / 1 naa , and the tall of other four buds was less 2 cm 篩選后的芽在1 / 2ms + naao . 1培養(yǎng)基上生長(zhǎng)40天后無(wú)根形成,并且6株芽中2株黃化死亡,其余4株高度不到2cm 。
Addition of img / l tdz and img / l naa resulted in the highest bud regeneration rate ( 67 . 9 % ) and number ( 35 ) of buds per explant . regeneration rate could be improved apparently in the medium with agno3 1mg ltdz和1mg lnaa配合對(duì)子葉再生具有極強(qiáng)的誘導(dǎo)作用,再生率最高,達(dá)到67 . 9 ,平均每外植體不定芽數(shù)達(dá)35個(gè)。
0 mg / 1 6ba . shoot buds were formed on subcultured pieces of callus with ms medium containing 0 . 3 mg / 1 naphtaleneacetic acid ( naa ) and 2mg / 16ba . the regenerated shoots rooted readily on ms medium plus 0 . 5 mg / 1 naa 研究發(fā)現(xiàn)精氨酸( arg ) 、甲硫氨酸( met ) 、絲氨酸蘇氨酸( ser thr )并不是黃精凝集素生物學(xué)活性的必需基團(tuán)。
It is easy to induce callus derived from hypocotyls and cotyledons , but callus derived from root tip was rather difficult to be done . the most suitable medium for callus induction was the ms medium containing ba 0 . 5 mg / l and l . omg / l naa 愈傷組織的誘導(dǎo)與再分化的研究顯示: “種都一號(hào)”兒菜下胚軸與子葉愈傷組織誘導(dǎo)較容易,但根尖很難愈傷化。
The enriched stable isotope tracer technique combined with neutron activation analysis and differential centrifugation was utilized to investigate the subcellular distribution of rare earth elements sm and yb in rat liver Sm和, sup 168 yb作為示蹤劑,結(jié)合差速離心分離和中子活化分析技術(shù)naa初步研究了稀土元素sm和yb在大鼠肝臟亞細(xì)胞組分中的分布。
High frequency of bud induction was found on ms medium supplemented with 6 - ba the optimum medium for adventitious bud induction was ms + l . 0mg / 1 naa + 0 . 5mg / l 6 - ba , and ms + 1 . 5mg / l naa + 0 . 5mg / l iaa for bud rooting 門(mén))誘導(dǎo)毛狀根體細(xì)胞胚再生植株。通過(guò)正交實(shí)驗(yàn)篩選出了毛狀根體細(xì)胞胚誘導(dǎo)的最佳培養(yǎng)基為: ms 05mg lnaa 0 sing kt 30gil蔗糖。
Naa can not only defend the invasion of various microorganism , but maintain the stability of internal envioment , lead to self tolerance , regulate immunity and prevent tumor progression 從不同角度對(duì)naa的研究顯示: naa的產(chǎn)生可能與自身反應(yīng)性b細(xì)胞克隆陽(yáng)性選擇過(guò)程有關(guān); naa對(duì)外可以抵御外來(lái)微生物的入侵;對(duì)內(nèi)可以維持機(jī)體內(nèi)環(huán)境的穩(wěn)定,引起自身耐受,調(diào)節(jié)免疫和抗腫瘤。
Among the three factors tested in our experiments , kt had the significant effect on the somatic embryo induction and its development , followed by naa sucrose had smallest effect on somatic embryo induction , but had the obvious effect T 、 a濃度和蔗糖對(duì)體胚的發(fā)育均有較明顯的作用。對(duì)體胚的發(fā)育最好在cling l的kt濃度下或不含激素的培養(yǎng)基中,蔗糖濃度最好在30g l 。