sequence n. 1.繼續(xù);接續(xù);連續(xù)。 2.順序;程序;次第;關(guān)系;關(guān)聯(lián)。 3.后果;結(jié)果;接著發(fā)生的事;后事;后文。 4.【數(shù)學(xué)】數(shù)列;序列;數(shù)貫。 5.【無、計】指令序列;定序。 6.【計算機】順序機〔將信息項目排成順序的機器〕。 7.【音樂】用不同音調(diào)反復(fù)演奏一組樂句。 8.【天主教】宣講福音前唱的圣歌。 9.【牌戲】順。 10.【電影】(描述同一主題的)連續(xù)鏡頭;片斷,插曲;場景。 a logical sequence 條理;邏輯順序。 a causal [physical] sequence 因果關(guān)系。 the natural sequence to [for] folly 愚笨行為的必然結(jié)果。 in rapid sequence 一個接著一個;緊接著。 in regular sequence 挨次;按次序;逐一;有條不紊地。 in sequence 挨次;順次;逐一。 sequence of tenses 【語法】時態(tài)的配合[接續(xù),呼應(yīng)]。
2 . firstly , the vector pcambia2301g with two gus reporter genes was constructed . then one gus was substituted with the coding sequence of dsg 10 obtained by pcr , and pcambia2301g - dsg10 was constructed . finally the pcambla2301g - dsg10 was introduced into agrobacterium tumefaciens using the freeze - thaw method 3 2 、首先構(gòu)建了中間載pcambia2301g ,再用它和dsg10構(gòu)建了可在植物中高效表達dsg10的載體pcambia2301g - dsg10 ,并將該質(zhì)粒導(dǎo)入根癌農(nóng)桿菌( agrobacteriumtumefaciens ) eha105中。
Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3 . the recombinant plasmid was transformed to e . coli bl21 and the gst / mpem fusion protein was induced to express with iptg . the fusion protein was purified by affinity chromatography 方法: pcr擴增小鼠pemcdna編碼序列,將它克隆到含有g(shù)st的原核表達質(zhì)粒載體pgex - 4t - 3上,轉(zhuǎn)化大腸桿菌bl21 ,誘導(dǎo)表達gst mpem融合蛋白,通過親和層析,獲得初步純化的產(chǎn)物,以羥胺切割驗證其一級結(jié)構(gòu)。
Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing 目的構(gòu)建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數(shù)據(jù)庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結(jié)合大腸桿菌蛋白質(zhì)合成體系對氨基酸密碼子使用的偏愛性,設(shè)計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當?shù)南拗菩詢?nèi)切酶位點插入表達載體pbv220 ,分別構(gòu)建了echistatin的單拷貝表達克隆、雙拷貝串聯(lián)表達克??;進一步通過pcr技術(shù)構(gòu)建echistatin的融合表達基因克隆。
Rlean meat percentage is one of the most important economic traints in pig breeding programs . myostatin is a negative regulator of skeletal muscle growth . null or low activity of myostatin , individual muscle of mutant amimals would show a large and widespread increase in skeletal mass . myostatin null animals have significantly larger diameter or more quantity of fiber skeletal muscle . the phenotype was termed double muscling . in order to probe the relation between myostatin and high lean meat rate and plump - hipped trait , we sythesized the c ' 80 amino acids coding sequence of porcine myostatin and costructed the cloning and expressing vector of it 肌生成抑制素( myostatin ,即mstn )是近幾年來( mcpherrona . c等, 1997 )發(fā)現(xiàn)的骨骼肌生長的負調(diào)控因子,它主要在骨骼肌中表達。其活性的喪失,會引起動物肌肉的過度發(fā)育,肌纖維直徑變大或肌纖維數(shù)增加,表現(xiàn)為雙肌癥狀。肌生成抑制素研究的突破將對豬、肉雞、肉牛等畜禽生產(chǎn)性能的提高具有特別重要的意義。
In addition , the nt - 3 and nt - 4 coding sequences were used to resolve the taxa problems of the giant panda and the red pandacaizurus fulgens ) , combining with mtdna genetic marker and other traditional taxonomic data . the result suggested that the giant panda should be classified as a separate subfamily within ursidae , while the red panda should be included in a monotypic family ailurdae 4基因的保守性,結(jié)合線粒體dna上的序列和其它傳統(tǒng)分類學(xué)資料,對大熊貓及小熊貓的分類地位進行了探討,認為應(yīng)將小熊貓單獨列為一科,大熊貓與小熊貓關(guān)系較遠,應(yīng)劃入熊科。
In the experiment , the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked . it was expressed in e . coli and its protein was determined . after having been properly modified , the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing , which is the prerequisite work for genetic transformation 本實驗從抗除草劑轉(zhuǎn)基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,并在原核表達系統(tǒng)中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉(zhuǎn)移酶的bar基因片段,經(jīng)過適當?shù)男揎棙?gòu)建入真核表達載體。