The suitable concentration of the selective agent kanamycin is 50mg . l - 1 . the duration of infection and co - culture with agrobacterium influences soybean transformation evidently 農(nóng)桿菌侵染時(shí)間和共培養(yǎng)時(shí)間對(duì)大豆的轉(zhuǎn)化有明顯的影響,侵染時(shí)間以15min為宜,共培養(yǎng)時(shí)間以3d為宜。