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sequence-specific中文是什么意思

  • 序列特異的

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  • 例句與用法
  • The back - exchange process is actually complex and deserving of analysis in a sequence - specific manner
    返交換實(shí)際上很復(fù)雜,需要進(jìn)行序列特異性的分析。
  • The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
    本研究設(shè)計(jì)以c tamrna為模板的反義cdna片段,從c ta基因5 ’端第94位到500位核苷酸段設(shè)計(jì)引物,目的片段407bp ,覆蓋第116和188位兩個(gè)aug密碼子,也包含了第一外顯子和第二外顯子間的剪接位點(diǎn):用常規(guī)分子生物學(xué)方法構(gòu)建了反義片段的腺病毒表達(dá)載體( padeasy - 1系統(tǒng)) ;腺病毒載體經(jīng)hek293細(xì)胞包裝產(chǎn)生含反義片段的重組腺病毒,用氯化銫密度梯度離心法獲得純化的高滴度腺病毒;進(jìn)行體外基因轉(zhuǎn)移,分別用反義片段真核表達(dá)載體轉(zhuǎn)染p388d1細(xì)胞和用重組腺病毒感染hela細(xì)胞,觀察導(dǎo)入的c ta基因反義rna抑制細(xì)胞內(nèi)組成型或誘導(dǎo)型c ta基因表達(dá)的作用,從而達(dá)到調(diào)控mhc -類分子表達(dá)的目的。
  • In this paper , a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied . in addition , we synthesized and analyzed a sequence - specific cleavage reagent , in which oligodeoxynucleotide was recognizing group . detailed work mentioned below were carried out : 1
    本文在查閱大量文獻(xiàn)的基礎(chǔ)上,以1 , 10 -鄰菲咯啉和1 , 10 -鄰菲咯啉- 5 , 6二酮為配體合成了一系列過渡金屬配合物,詳細(xì)研究了它們與pbr322dna的相互作用及其機(jī)理。
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