插入 insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromission; dig; implant; inlay; interpose; intervene; interposition [數(shù)學(xué)] interpolation 把鑰匙插入鎖中 insert a key in(to) a lock; 用劍插入某人的胸膛 thrust one's sword through sb.'s bosom; 有時(shí)插入一個(gè)字可以改變?nèi)涞囊饬x。 sometimes the insertion of one word can change the meaning of a whole sentence
Transformation efficiency is greatest with circular plasmids containing only small inserts of passenger dna . 使用帶有一小段客座DNA的插入序列的環(huán)狀質(zhì)粒進(jìn)行轉(zhuǎn)化,較率會(huì)最高。
Transformation efficiency is greatest with circular plasmids containing only small inserts of passenger dna 使用帶有一小段客座dna的插入序列的環(huán)狀質(zhì)粒進(jìn)行轉(zhuǎn)化,較率會(huì)最高。
The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod 結(jié)果表明同源性分別達(dá)到98和97 ,并且在ha1切割位點(diǎn)有多個(gè)堿性氨基酸的插入序列,證明其為強(qiáng)毒株。
Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis . we took a pcr method to clone the cdna of osdd2 gene 使用克隆的邊端插入序列為探針對(duì)水稻(中花11品種)進(jìn)行southern雜交分析,表明osdd2基因在水稻基因組中是以單拷貝存在。
Methods : the two pairs of designed primers were based on pzp3 a and hcg p - ctp109 - 145 cdna sequences . the pzp3 a - hcg p - ctp109 - 145 chimera was amplifiled by overlapping pcr . the chimera was cloned into ppic9k plasmid and transformed into e . coli dh5 a 結(jié)果: 3步pcr擴(kuò)增出pzp3 - hcg - ctpdna片段,插入到載體質(zhì)粒ppic9k的克隆位點(diǎn),獲得重組ppic9k - pzp3 - hcg - ctp表達(dá)質(zhì)粒,測(cè)序結(jié)果顯示插入序列與設(shè)計(jì)預(yù)期完全一致。